The new biological preservation technology has the incomparable advantages, such as less pollution and low cost, compared with the conventional fresh-keeping technology. This paper mainly reviews several techniques frequently used in fruits and vegetables preservation. Firstly, the principle of antagonistic bacteria preservation technology and its application are discussed. Secondly, gene engineering preservation technology is mainly discussed from aspects of reducing the generation of ethylene and regulating the activity of cell wall-degrading enzymes. Thirdly, enzyme engineering preservation technology is also reviewed from the view of enzyme preparation.