几种植物淀粉合成酶的生物信息学分析
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Q559

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Bioinformatics Analysis of Starch Synthase in Several Plants
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    摘要:

    采用生物信息学方法比对分析了马铃薯、红薯、小麦、高粱、南瓜、水稻等6种植物的淀粉合成酶(SS)的核苷酸和氨基酸序列,对其甲基化位点、组成成分、理化性质、疏水性/亲水性、跨膜结构域、蛋白质二级结构、功能结构域进行预测和分析。结果表明:红薯、小麦、高粱和水稻都含有甲基化位点,马铃薯和南瓜不含甲基化位点。SS的平均全长为 1 847 bp,开放阅读框的长度约为 877 bp,起始密码子为ATG,终止密码子有3种;开放阅读框所编码的氨基酸残基平均数 286,平均相对分子质量为31 915.88;平均等电点为 8.264 5,中性溶液中平均带电荷为-0.598 1;平均亲水氨基酸 63个、疏水氨基酸 102个,SS为疏水性蛋白。6种植物的SS含有2个跨膜结构,其二级结构以无规则卷曲和延伸链为主要构件。通过进化树分析淀粉合成酶,小麦和高梁进化程度相似,和南瓜的进化相距较远。

    Abstract:

    The nucleotide and amino acid sequences of starch synthase gene(SS) of six plants(Solanum tuberosum,Impomoea batatas,Triticum aestivum,Sorghum bicolor,Cucurbita moschata,Oryza sativa) were analysed using bioinformatic method and the physical and chemical properties and functional domains were predicted. The results showed that SS cDNAs of S. tuberosum,I. batatas,T. aestivum and S. bicolor contained methylation site. The average length of SS was 1 847 bp and the average length of the open reading frame was 877 bp. The start codons were all ATG,and the stop codon consists of 3 kinds. The average number of amino acid residues was 286,and the average relative molecular mass was 31 915.88,the average isoelectric point was 8.2645,the average charge was -0.598 1 in neutral solution. The average number of hydrophilic amino acid was 63,and the average number of hydrophobic amino acid was 102. All the SS of these six plants had two transmembrane domains.The irregular curl and extension chain were found the main component of all the SS secondary structures. Throngh starch synthase by phylogenetic analysis,T.aestivum was similar with S. bicolor in degree of evolution ,but far away from C. moschata.

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田亚珍,武国凡,秦绪军,牛世全,孔维宝.几种植物淀粉合成酶的生物信息学分析[J].食品与生物技术学报,2018,37(9):948-954.

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  • 在线发布日期: 2018-10-30
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