Henry Fok School of Biology and Agriculture, Shaoguan University
To explore the effects of soybean peptides from soybean protein isolate(SPI) hydrolyzed by the recombinant Aspergillus sojae alkaline protease (rAp) on immunity function and antioxidation in mice. In order to produce the rAp, the recombinant P. pastoris strain was constructed and inoculated in shake flask. SPI was hydrolyzed by the rAp, and then the soybean peptides were obtained. Furthermore, the immunity functions of the soybean peptides was to investigate the effects of the soybean peptides on immune organ indices, serum lysozyme activity and the anti-bacterial activity. And the antioxidation of soybean peptides was to determine the level of malondialdehyde(MDA), the activities of glutathione peroxidase (GSH-Px) and catalase(CAT). The results indicated that the immune organ indices had no remarkable change, but the serum lysozyme activity and the anti-bacterial activity were enhanced. In addition, the content of MDA was significantly lower to the control group. The activities of GSH-Px and CAT were significantly higher to the control group. In conclusion, the soybean peptides can enhance the immune function and anti-oxidative activities in mice.