荞麦蜂花粉多糖A的分离及其降血糖活性
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O629.12

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Separation of Polysaccharide A from Buckwheat Bee Pollen and Its Hypoglycemic Activity
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    摘要:

    对荞麦蜂花粉粗多糖进行分离鉴定并检测纯化多糖的降血糖活性。利用DEAE-52离子交换柱、HW-55F、Sephacryl S-400凝胶柱对荞麦蜂花粉粗多糖进行分离,得到一个单一组分多糖A。利用STZ糖尿病小鼠模型检测多糖A的降血糖活性并通过核磁方法对其进行1H-NMR、13C-NMR和135DEPT-NMR分析。结果表明:与高血糖模型组相比,多糖A高、低剂量组的STZ糖尿病小鼠空腹血糖值都显著降低(P<0.01,P<0.01),降低率分别为18.46 %、15.11 %,并提高糖尿病小鼠糖耐量。初步推测多糖A主链是(1→6)-β-葡萄糖组成的,在侧链上连有氨基酸。

    Abstract:

    The polysaccharides from buckwheat bee pollen were isolated and identified,and the hypoglycemic activity of purified polysaccharides was determined. By using DEAE-52 ion-exchange column,HW-55F gel column and Sephacryl S-400 gel column,polysaccharide A was separated and purified from the crude polysaccharides. The hypoglycemic activity of polysaccharides A was evaluated through the mouse model of diabetes and polysaccharides A was identified by using nuclear magnetic resonance technique. Compared with control group(without treatment of polysaccharide A),the blood glucose in diabetic mice was notably decreased with treatment of polysaccharide A at both high and low doses,with decreased rates of 18.46% and15.11%,respectively. Moreover,polysaccharide A improved glucose tolerance in diabetic mice and its main chain was deduced to be composed of (1 → 6) -β- glucose and the side chains are linked with amino acids.

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钱明辉,达热卓玛,徐德平.荞麦蜂花粉多糖A的分离及其降血糖活性[J].食品与生物技术学报,2019,38(6):80-85.

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  • 在线发布日期: 2020-01-07
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