沙门氏菌污染豆腐干和牛肉干后代谢物的研究
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Study on Metabolites of S. typhimurium Polluted Dried Bean Curd and Beef Jerky
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    摘要:

    鼠伤寒沙门氏菌可引发沙门氏菌病,且耐药性强,严重危害人畜健康。利用气相色谱-质谱法(Gas Chromatography - Mass Spectrometer,GC-MS)显示沙门氏菌接种于NB培养基、豆腐干和牛肉干培养基后的代谢物,采用主成分分析和正交偏最小二乘判别鉴别特征性和差异性代谢产物,结合沙门氏菌的基因注释分析特征性和差异性代谢产物可能的代谢路径。结果表明:沙门氏菌污染两种食品后有显著的生长,特征性和差异性代谢产物包括烷烃,酚类及小分子酸、醇等;沙门氏菌的基因注释表明存在大量的碳水化合物代谢基因,参与了1-丁醇的生成,因此1-丁醇是沙门氏菌污染豆腐干和牛肉干的典型代谢物。

    Abstract:

    Salmonella typhimurium (S. typhimurium) can cause salmonellosis and is highly drug- resistant, causing seriously hazards on the health of both human and animals. The metabolites of S. typhimurium inoculated on NB medium, dried bean curd and dried beef medium were detected by Gas Chromatography-Mass Spectrometer(GC-MS). Principal component analysis (PCA) and orthogonal partial least squares discrimination analysis(OPLS-DA) were used to identify the characteristic and differential metabolites. The probably metabolic pathways of the characteristic and differential metabolites were analyzed based on the gene annotation of S. typhimurium. Significant growth of S. typhimurium was observed after inoculated dried bean curd and dried beef. The characteristic and differential metabolites were alkanes, phenols, small molecular acids, small molecular alcohols, etc. Gene annotation of S. typhimurium showed a large number of carbohydrate metabolism genes, involved in the production of 1-butanol. Therefore,1-butanol is a typical metabolite of S. typhimurium polluted dried bean curd and beef jerky.

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胡蝶,胡昊,杨本芹,潘学军.沙门氏菌污染豆腐干和牛肉干后代谢物的研究[J].食品与生物技术学报,2020,39(10):104-111.

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  • 在线发布日期: 2021-01-15

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