人参皂苷Rg1对疲劳小鼠骨骼肌中TFAM和NRF-1基因表达的影响
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黑龙江八一农垦大学食品学院

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黑龙江省科技厅自然基金研究团队项目(TD2020C003);黑龙江省应用技术研究与开发计划重大项目(“南病北治,北药南用”专项)(GY2017YD0215);国家自然科学基金面上项目(81673170)


Effects of Ginsenoside Rg1 on Gene Expression of TFAM and NRF-1 in Skeletal Muscle of Fatigued Mice
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College of Food Science, Heilongjiang Bayi Agricultural University

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    研究人参皂苷 Rg1 对疲劳模型小鼠抗疲劳作用及骨骼肌中线粒体转录因子 A(TFAM)和核呼吸因子-1(NRF-1)基因表达的影响。选取 70 只雄性 BALB/c 小鼠随机分为七组,除空白组外,小鼠腹腔注射环磷酰胺,造成免疫低下模型后进行小鼠负重游泳力竭实验,ELISA 法测定小鼠肝脏及骨骼肌中疲劳相关指标的变化情况,RT-PCR 法检测小鼠骨骼肌中 TFAM 和 NRF-1 基因表达水平。结果表明,人参皂苷 Rg1 中剂量组小鼠负重游泳的力竭时间最长,较空白组增加了 271.4%,且能显著增加肝糖原含量。各剂量组小鼠肝脏 MDA 水平下降,LDH 水平升高。中剂量人参皂苷 Rg1 可显著增加小鼠骨骼肌中 TFAM mRNA 和 NRF-1mRNA 表达水平。人参皂苷 Rg1 可有效调节疲劳小鼠骨骼肌中疲劳相关生化指标的水平,具有较好的抗疲劳效果。

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    To investigate the anti-fatigue effect of ginsenoside Rg1 on fatigue model mice and the influence on mitochondrial transcription factor A (TFAM) and nuclear respiratory factor-1 (NRF-1) gene expression in skeletal muscle. Seventy male BALB/ C mice were randomly divided into seven groups. In addition to the blank group, mice were injected intraperitoneally with cyclophosphamide, which caused immunodeficiency model. And then the mice were subjected to the full-load swimming exhaustion experiment. Determination of changes in fatigue-related indexes in liver and skeletal muscle of mice by ELISA. The expression levels of TFAM and RF-1 genes in the skeletal muscle of mice were determined by RT-PCR. The results showed that the duration of weight-bearing swimming was the longest in the middle dose group of ginsenoside Rg1, which increased by 271.4% compared with the blank group. The content of liver glycogen was significantly increased. The level of MDA and LDH in liver of mice in each dose group decreased. Medium doses of ginsenoside Rg1 could significantly increase the expression levels of TFAM mRNA and NRF-1mRNA in the skeletal muscles of mice. Ginsenoside Rg1 can effectively adjust the biochemical indicators related in skeletal muscle fatigue. So ginsenoside Rg1 has better anti-fatigue effect.

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  • 收稿日期:2020-11-11
  • 最后修改日期:2021-03-19
  • 录用日期:2021-04-01
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