黄芪多糖对黄嘌呤氧化酶活性的抑制作用
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内蒙古医科大学

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内蒙古医科大学第一临床医学院2020年大学生“启蒙计划”(FYQMJH2020037);国家自然科学基金(32050217);内蒙古科技计划(2019GG160);内蒙古自然科学基金项目(2019BS08013);内蒙古自治区高等学校科学研究项目(NJZY19100);内蒙古蒙医药协同创新项目(MYYXT201901);内蒙古医科大学人才团队项目(NYTD-2018003)资助


Inhibitory Effects of Astragalus Polysaccharideon Activity of Xanthine Oxidase
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Inner Mongolia Medical University

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    摘要:

    黄嘌呤氧化酶是尿酸生成的关键酶,通过抑制黄嘌呤氧化酶的活性可以有效降低机体内尿酸的含量。为探究黄芪多糖对黄嘌呤氧化酶的抑制作用,采用紫外分光光度法测定单位时间内酶促反应体系中尿酸含量的变化,计算不同浓度的黄芪多糖对黄嘌呤氧化酶的抑制率,得到半抑制浓度IC50。双倒数作图法判断黄芪多糖对黄嘌呤氧化酶为何种抑制类型,并计算抑制常数Ki值,等毒法评价黄芪多糖与别嘌呤醇的联合作用效果。黄芪多糖能够抑制黄嘌呤氧化酶的活性,其IC50为1.37 mg/mL。动力学分析表明,黄芪多糖对黄嘌呤氧化酶为竞争性可逆抑制作用,抑制常数Ki为0.59 mg/mL。黄芪多糖与别嘌呤醇的联合作用表现为拮抗作用。黄芪多糖对黄嘌呤氧化酶的活性有抑制作用。

    Abstract:

    Xanthine Oxidase is a key enzyme in the formation of uric acid, and the content of uric acid in vivo can be effectively reduced by inhibiting the activity of Xanthine Oxidase. To explore the inhibitory effects of Astragalus Polysaccharide on Xanthine Oxidase, the variation of uric acid content of reaction system per unit time was determined by UV-Spectrophotometry, and the inhibition ratio was calculated and the IC50 was obtained. The inhibition type of Astragalus Polysaccharide on Xanthine Oxidase was determined by drawing a Lineweaver-Burk plot, and a Dixon plot was drawn to obtain the inhibition kinetic parameter (Ki). The inhibitory effects of Astragalus Polysaccharide combining allopurinol were explored by toxicity method. Astragalus Polysaccharide could inhibit the activity of Xanthine Oxidase, and the IC50 was 1.37 mg/mL. Dynamic analysis showed that Astragalus Polysaccharide was competitive reversible inhibitor for Xanthine Oxidase , with an inhibition contant (Ki) of 0.59 mg/mL. There was antagonism effect when Astragalus Polysaccharide combined allopurinol. Astragalus Polysaccharide could inhibit the activity of Xanthine Oxidase.

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  • 收稿日期:2020-12-02
  • 最后修改日期:2021-03-30
  • 录用日期:2021-04-05
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