Yunnan agricultural university,University,Kunming,Yunnan,China
本文研究了绿原酸对UVB损伤HaCaT细胞的保护作用及机制,旨为富含绿原酸的食品资源开发利用提供科学依据。通过MTT法建立细胞紫外损伤模型及绿原酸的处理方式,酶标仪检测绿原酸的紫外吸收,流式细胞术检测细胞的凋亡水平,ABTS法检测绿原酸的自由基清除能力。结果表明：UVB辐射剂量为21.6mJ/cm2,绿原酸浓度为100和150μmol/L,预处理1h后细胞培养12h；绿原酸在UVB波段(280nm-320nm)范围有较强的吸收；100和150 μmol/L 绿原酸均能显著降低UVB引起的细胞凋亡；150 μmol/L 绿原酸能有效增加UVB处理细胞的ABTS+自由基的清除能力,达到了53%。因此绿原酸在UVB诱导的HaCaT光老化模型上,具有很强的抗皮肤光老化活性,其机制可能与绿原酸的紫外吸收以及抗氧化能力相关。
In order to provide scientific basis for the development and UV of food resources rich in chlorogenic acid, the protective effect and mechanism of chlorogenic acid on HaCaT cells injured by UVB were studied. MTT method was used to establish cell UV damage model and chlorogenic acid treatment method. The UV absorption of chlorogenic acid was detected by enzyme labeling instrument. The apoptosis level of cells was detected by flow cytometry. The free radical scavenging ability of chlorogenic acid was detected by ABTS method. The results showed that the radiation dose of UVB was 21.6mJ/cm2, and the concentration of chlorogenic acid was 100 and 150 μmol/L After pretreatment for 1 h, the cells were cultured for 12 h; Chlorogenic acid has strong absorption in UVB band (280nm-320nm); 100 and 150 μmol/L chlorogenic acid both significantly reduced UVB induced apoptosis; 150 μmol/L chlorogenic acid could effectively increase the ABTS+ free radical scavenging ability of UVB treated cells by 53%. Therefore, chlorogenic acid has strong anti skin photoaging activity in HaCaT photoaging model induced by UVB, and its mechanism may be related to the UV absorption and antioxidant capacity of chlorogenic acid.