Abstract:To explore the inhibitory effect of extract of Lucid Ganoderma total saponins (LGTS) on colon cancer HCT116 cells and related mechanisms. Ganoderma lucidum fruiting bodies were used as raw material, the LGTS was extracted by alcohol and then purified. The inhibitory effect of LGTS on colon cancer HCT116 cells was detected by CCK8 method. Meanwhile, the state of apoptosis was detected by flow cytometry, intracellular mitochondrial membrane potential and reactive oxygen content in cells were recorded by fluorescence spectrophotometer, and the expression of apoptosis-related genes such as p53, noax, bax, bcl-2, caspase-9 and caspase-3 were measured by real-time fluorescent quantitative PCR. The results showed that compared with the blank group, the concentration of LGTS above 40 μg/mL could remarkably inhibit the proliferation of HCT116 cells, and the inhibitory effect was positively correlated with time. Through the intervention of GLTS, the apoptosis rate increased notably. While the concentration of GLTS was 150 μg/mL, the apoptosis rate reached 50.2%, the intracellular mitochondrial membrane potential was significantly reduced, reactive oxygen content was increased, the expression of pro-apoptotic genes (p53, noax, bax, caspase-9 and caspase-3) were upregulated, the expression of anti-apoptotic gene (bcl-2) was downregulated. On the whloe, GLTS had an important inhibitory effect on colon cancer cells which can be achieved through the mitochondrial apoptosis pathway. As a candidate drug and food additive for colon cancer it deserves further research.
