Abstract:The effects of γ-oryzanol and cysteamine on the redox status and immunity of stressed mice were investigated. Ninety male ICR mice aged five weeks were randomly divided into 6 groups with 15 mice in each group. Stressed mouse model was developed by glucocorticoid injection, meanwhile, different doses of γ-oryzanol and cysteamine were added to the feed for 8-week administration. Compared with the model group, the activity of total superoxide dismutase (SOD) in tissues, the activity of glutathione peroxidase (GSH-Px) and the total antioxidant capacity (T-AOC) in plasma and tissues of the combination group 1 were significantly increased (P<0.05), while the malondialdehyde (MDA) content in plasma and tissues was significantly decreased (P<0.05). The activity of Mn-SOD, membrane potential, acetyl coenzyme A (A-CoA), and ATP in the mitochondria of combination group 1 were significantly increased (P<0.05), while the mitochondrial MDA content was significantly decreased (P<0.05). The spleen index, thymus index and the content of immunoglobulin in the plasma of combination group 1 were significantly increased (P<0.05). The levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were significantly decreased (P< 0.05), while the level of interleukin-10 (IL-10) was significantly increased (P<0.05). The results showed that the redox status and immune function of stressed mice were improved in the four experimental groups, and the combination group 1 had the most significant effect, indicating that the combination of γ-oryzanol and cysteamine at a lower dose could play a synergistic role in anti-oxidation and regulating immune function, thus effectively resisting the harm caused by oxidative stress.
