Abstract:Xylan is the most abundant heterogeneous polysaccharide in plant cell walls, which has great potential to synthesize high-value bio-based products. In the food industry, efficient enzymatic conversion of xylan can produce functional food polysaccharides such as xylo-oligosaccharides, xylitol and ferulic acid. Compared with the multi-enzyme synergistic catalysis, multifunctional enzymes combining multiple xylanolytic activities in one single peptide have significant advantages in reducing enzyme costs, improving catalytic efficiency and simplifying the enzymatic process, and therefore have broad application prospects. This paper firstly introduced the structural composition of xylan from different plant sources and the enzymatic systems for the degradation of main and side chains, then systematically reviewed the research progress of multifunctional xylanolytic enzyme with respect to structure, function and mechanism of action and their potential applications in the food industry, and finally prospected the research direction of multifunctional xylanolytic enzymes, laying a foundation for their precise design and industrial application.