为了探寻一种多模式超声高效促进克氏原螯虾酶解效果的方法，分别采用双频探头式超声波和三频发散式超声波对克氏原螯虾进行预处理，对比研究了多模式超声预处理对克氏原螯虾酶解效果的促进作用。通过单因素实验得到探头式双频超声设备的最佳频率模式为20/28 kHz双频顺序超声，三频发散式超声波设备的最佳频率为20/40 kHz双频顺序超声。在此基础上，通过三因素三水平的响应面实验对超声预处理条件进行改进，并建立相应回归方程，优化试验以获得最佳的超声预处理条件。结果表明：双频探头式超声设备的最佳处理条件为：超声功率为250 W，时间为10 min，温度为35 ℃，此时克氏原螯虾的水解度为38.98%；三频超声波设备的最佳处理条件为：超声功率为250 W，时间为40 min，温度为40 ℃，此时克氏原螯虾的水解度为46.12%。实验结果表明多模式超声可以有效提高克氏原螯虾酶解效果。
In order to explore a high-efficient method to promote the enzymatic hydrolysis of Procambarus clarkia with multi-mode ultrasound, the dual-frequency probe ultrasound and tri-frequency divergent ultrasound were used to pretreat Procambarus clarkii in this study, and the promotion effects of multi-mode ultrasonic pretreatment on the enzymatic hydrolysis of Procambarus clarkii were comparatively investigated. Through the single factor experiments, the optimal frequency mode of the dual-frequency probe ultrasonic equipment was found to be 20/28 kHz in the sequential mode, while that of the three-frequency divergent ultrasonic equipment was 20/40 kHz in the sequential mode. On this basis, the response surface experiment with three factors and three levels was carried out to optimize the ultrasonic pretreatment conditions and to establish a corresponding regression equation. The optimization experiment was conducted to obtain the best ultrasonic pretreatment conditions. The optimal pretreatment conditions of the dual-frequency probe ultrasonic equipment were determined, including the use of 250 W ultrasonic power, treatment time of 10 minutes, and temperature of 35 ℃. As a result, the degree of hydrolysis of Procambarus clarkii reached 38.98%. Similarly, the tri-frequency ultrasonic equipment achieved an optimal hydrolysis rate of 46.12% under the optimal conditions of 250 W ultrasonic power, 40 minutes duration, and 40 ℃. The experimental results showed that multi-mode ultrasound could effectively improve the enzymatic hydrolysis effect of Procambarus clarkii.
ZHANG Chao, HAN Chang, FENG Min, RUAN Wenyan, YANG Haoze, XU Baoguo, YAN Weiqiang, ZHOU Cunshan, MA Haile. Optimization of Enzymatic Hydrolysis of Procambarus clarkia via Multi-Mode Ultrasonic Pretreatment[J]. Journal of Food Science and Biotechnology,2023,42(10):51-62.