Abstract:To achieve the double-win of economic and environmental benefits, how to increase the degradation rate of protein in the wastewater and to reduce the odor substances that generated during the fermentation process in the treatment of the wastewater of potato starch processing were studied. The aerobic Pseudomonas bryophyta and anaerobic Serratia marcescens were mixed at a low temperature (15 ℃) and the fermentation conditions were optimized by single factor and response surface methodology. Results showed that the optimum processing conditions were: the inoculum amount 1.98%, mass ratio of Pseudomonas bryophyta to Serratia marcescens 1:2, fermentation time 48 h, the protein degradation rate was 65.32% in this condition. Compared with the group without fermentation, ammonia nitrogen content decreased by 51.05%, and the phosphate content increased by 18.67%. Volatile malodorous substances such as indole, hydrogen sulfide and 3-methylindole were undetectable, so was the biogenic amines such as cadaverine, histamine, tryptamine, tyramine, spermine, and spermidine. Moreover, the content of growth hormones like indole acid-valine methyl ester and indole acetic acid oxide increased during fermentation, which was beneficial to the germination of cucumber seeds. The fermentate method proposed can economically solve the problem of wastewater treatment of potato starch processing at low temperature environment, and provide a certain basis for subsequent volarization of fermented potato wastewater.