In order to establish a rapid detection method for carrot components in complementary foods for infant and young children using isothermal real-time fluorescence, specific primers for loop-mediated isothermal amplification (LAMP) were designed and synthesized based on the carrot ITs1-5.8 SRNA-ITS2 gene sequence. The specificity and sensitivity of the method were verified by DNA from common crop and white radish with different carrot mass ratios. Different cultivars of carrot seeds were selected to verify the applicability of the method, and its practicality was tested through pre-packaged food. The results indicated that the established method exhibited strong specificity without cross-reactivity with other species. It can detect the composition of carrot as low as 0.01% mass ratio within 1h, demonstrating high sensitivity. It is suitable for the detection of different carrot cultivars with a wide range of application. The method proved to be fast and accurate when validated with actual samples. This method offers simplicity, cost-effectiveness, real-time synchronization of intelligent data interpretation, and accurate and rapid visualization of results. Therefore, it can be used for the rapid detection of carrot components in complementary foods for infant and young children, providing a technical basis labeling management and food safety supervision in complementary foods for infant and young children.
NIU Huimin, JIANG Jing, HU Hanwen, WANG Jingyi. Study on Rapid Determination of Carrot Components in Complementary Foods for Infant and Young Children by Isothermal Real-Time Fluorescence Assay[J]. Journal of Food Science and Biotechnology,2023,42(10):94-99.