自然发酵刺梨果渣中酵母菌的筛选及其特性研究
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贵阳学院食品科学与工程学院,贵州 贵阳 550005

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李勇(1986—),男,博士,副教授,硕士研究生导师,主要从事传统发酵食品微生物资源发掘和利用研究。E-mail:yongL33@126.com

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贵州省教育厅青年科技人才成长项目(黔教合KY字[2022]002号);2023年贵阳学院硕士研究生科研基金项目(2023-YJS02)。


Screening and Characterization of Yeast Strains from Naturally Fermented Rosa roxburghii Pomace
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School of Food Science and Engineering, Guiyang University, Guiyang 550005, China

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    摘要:

    【目的】为开发优质刺梨(Rosa roxburghiiR. roxburghii)发酵产品,筛选适于刺梨发酵的酵母菌株。【方法】以自然发酵刺梨果渣为材料,通过TTC显色初筛和发酵能力复筛,SO2、乙醇、低pH和葡萄糖耐受性试验,以及形态学和分子生物学方法对菌株进行鉴定。【结果】最终获得3株性能突出的菌株(B17、B19、B29),3株菌可耐受250 mg/L 的SO2、质量分数60%的葡萄糖、体积分数8%的乙醇及pH 3.5的环境。经鉴定,B17、B29 为库德里亚毕赤酵母(Pichia kudriavzevii),B19为马克斯克鲁维酵母(Kluyveromyces marxianus),产酯量分别为4.11 、4.31、3.93 g/L。【结论】筛选出的菌株具备工业化生产刺梨发酵制品的潜力,尤其适用于开发低乙醇、高风味的果酒产品。

    Abstract:

    [Objective] This study aimed to develop high-quality fermented Rosa roxburghii (R. roxburghii) products by screening yeast strains suitable for R. roxburghii fermentation. [Method] The naturally fermented R. roxburghi pomace was used as the material for preliminary screening through the TTC colorimetric method and secondary screening based on fermentation capacity. The tolerance of strains to SO2, ethanol, low pH, and glucose was examined. The strains were identified via morphological characterization and molecular biological techniques. [Result] Three high-performing strains (B17, B19, and B29) were obtained. These strains exhibited tolerance to 250 mg/L SO2, mass fraction 60% glucose, volume fraction 8% ethanol, and pH 3.5 environment. B17 and B29 were identified as Pichia kudriavzevii, while B19 was characterized as Kluyveromyces marxianus, showing ester production of 4.11, 4.31, and 3.93 g/L, respectively. [Conclusion] The screened strains exhibit the potential for industrial production of fermented R. roxburghii products, particularly suitable for crafting low-alcohol, high-flavor fruit wine.

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李勇,袁芳,付余,彭罗.自然发酵刺梨果渣中酵母菌的筛选及其特性研究[J].食品与生物技术学报,2025,44(8):79-86.

LI Yong, YUAN Fang, FU Yu, PENG Luo. Screening and Characterization of Yeast Strains from Naturally Fermented Rosa roxburghii Pomace[J]. Journal of Food Science and Biotechnology,2025,44(8):79-86.

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  • 收稿日期:2025-03-20
  • 最后修改日期:2025-04-18
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  • 在线发布日期: 2025-11-26
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