Abstract:Due to high antioxidant activity,many foodstuffs and beverages such as fruits,vegetables,tea,and red wine were exhibited the excellent beneficial influence on human health.With increasing interest in the function and diversity of antioxidants in foods,several in vitro methods for measuring antioxidant activity of food,beverages and biological samples have been developed.The most commonly assay methods include oxygen radical absorbance capacity(ORAC),reducing power,determination of total phenols,2,2-azino-di-(3-ethylbenzothialozine-sulphonic acid)(ABTS),2,2-diphenyl-1-picrylhydrazyl(DPPH),hydroxyl radical-scavenger activity,superoxide radical-scavenger activity and lipid peroxidation inhibition.These methods differ in terms of their assay principles and experimental conditions.This review describes the methodology for assessing in vitro antioxidant capacity,including the principles,characteristics,merits and drawbacks of every method.In addition,their current status and problems in practical application are critically evaluated.
