紫芝子实体活性多糖的纯化和结构解析
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

Q53

基金项目:


Purification and Structure Elucidation of Bioactive Polysaccharide from the Fruiting Bodies of Ganoderma sinense
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    以刺激RAW264.7细胞释放NO活性模型为指导,通过乙醇沉淀和DEAE阴离子交换柱层析,从紫芝子实体中分离得到均一多糖GS30S1-20E,采用红外光谱、核磁共振、气相色谱-质谱法和高效液相等现代仪器,研究了GS30S1-20E的结构特性。结果表明:GS30S1-20E是一种β-D-葡聚糖,其重均相对分子质量为2.418×106,数均相对分子质量为1.668×106,多分散指数为1.450,特性粘度为927.49 mL/g,以β-(1,3)-糖苷键为主链和β-(1,6)-糖苷键为支链,主链与支链上糖残基的摩尔比为3∶1,具有刺激巨噬细胞释放NO的活性。

    Abstract:

    Crude polysaccharide GS30 precipitated from hot water extracts of the fruiting bodies of Ganoderma sinense were separated by DEAE-column chromatography,and then treated with ethanol precipitation to get a purified polysaccharide fraction GS30S1-20E. Modern analytical techniques,such as IR,NMR,GC/MS and HPSEC were conducted to analyze the structural characteristics of GS30S1-20E. Results showed that GS30S1-20E was a β-D-glucan with a high weight average molecular mass of 2.418×106 and number average molecular weight of 1.668×106,polydispersity of 1.450 and large viscosity of 927.49 mL/g. GS30S1-20E contains a backbone of(1→3)-linked-β-D-glucopyranosyl residues,bearing branches at the O-6 position of every three sugar residues along the backbone. An in vitro immunomodulating activity assay revealed that GS30S1-20E could significantly increase the release of NO from RAW 264.7 cells.

    参考文献
    相似文献
    引证文献
引用本文

应一君,刘艳芳,张劲松,唐庆九,杨焱,周帅,颜梦秋,张忠,韩伟.紫芝子实体活性多糖的纯化和结构解析[J].食品与生物技术学报,2019,38(1):133-138.

YING Yijun, LIU Yanfang, ZHANG Jingsong, TANG Qingjiu, YANG Yan, ZHOU Shuai, YAN Mengqiu, ZHANG Zhong, HAN Wei. Purification and Structure Elucidation of Bioactive Polysaccharide from the Fruiting Bodies of Ganoderma sinense[J]. Journal of Food Science and Biotechnology,2019,38(1):133-138.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2019-03-18
  • 出版日期:

版权所有:《食品与生物技术学报》编辑部

地址:江苏省无锡市蠡湖大道1800号  邮政编码:214122

电话:0510-85913526  电子邮件:xbbjb@jiangnan.edu.cn

技术支持:北京勤云科技发展有限公司

微信公众号二维码

手机版网站二维码