漆酶降解梗丝中木质素工艺条件的优化
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TS452.2

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Optimization of the Process Conditions for the Degradation of the Lignin in the Cut Stem By Laccase
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    摘要:

    采用3因素5水平的正交实验方法研究了不同酶解时间、酶解温度和漆酶用量对梗丝中木质素降解率的影响,同时对漆酶处理前后梗丝的化学成分和填充值进行测量,结果表明:不同酶解时间、酶解温度和漆酶用量极显著影响着木质素的降解率。使用漆酶降解梗丝中木质素的最佳参数是:酶解时间4 h,酶解温度50 ℃,漆酶用量10.0 ?滋L/g。在该参数条件下,梗丝中的木质素降解效果最好,降解率高达47.3%。使用漆酶处理梗丝后,除了木质素之外,梗丝的其它化学成分含量基本不发生变化,填充值也基本没有影响。在最优的参数条件下,使用漆酶能在不降低梗丝原有品质的前提下有效的降解梗丝中的木质素,提高梗丝的品质和可用性。

    Abstract:

    The experiment uses three factors five levels of orthogonal experimental method to study the different digestion times,enzymolysis temperatures and dosage of laccase influence on cut stem in lignin degradation rate. the change of chemical composition and filling values in cut stem before and after processing also have been measured. The result showed that:(1) Digestion time,enzyme solution temperature,dosage of laccase in cut stem have significantly effected on the degradation of lignin. The best parameter of the enzymatic hydrolysis is time 4 h,enzymolysis temperature 50 ℃,the dosage of laccase 10.0 ?滋L/g. Under this parameter,the lignin degradation effect is best and the degradation rate is as high as 47.3%.(2) The chemical composition of cut stem don't change after the treatment of laccase except lignin and the same situation appeared on the filling valuses. Under the condition of optimal parameters,the use of laccase can effectively degrade lignin,raising the quality and availability of cut stem,and don't lower the quality of cut stem.

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李晓,王宇,姚二民,丁美宙,晋照普,李全胜,刘强,顾小燕.漆酶降解梗丝中木质素工艺条件的优化[J].食品与生物技术学报,2019,38(1):156-159.

LI Xiao, WANG Yu, YAO Ermin, DING Meizhou, JIN Puzhao, LI Quansheng, LIU Qiang, GU Xiaoyan. Optimization of the Process Conditions for the Degradation of the Lignin in the Cut Stem By Laccase[J]. Journal of Food Science and Biotechnology,2019,38(1):156-159.

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  • 在线发布日期: 2019-03-18
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