重组Cystatin对冷藏鲢鱼的抑菌效果
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

TS254.4

基金项目:


Study on Bacteriostasis of the Cystatin from Chilled Silver Carp
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    分离鉴定4 ℃有氧冷藏鲢鱼去皮肉片货架期终点腐败相关优势假单胞菌,并研究鲢鱼重组Cystatin(家族II)对优势腐败假单胞菌的抑菌效果。首先利用CFC培养基从4 ℃有氧冷藏鲢鱼去皮肉片货架期终点,分离筛选出腐败相关假单胞菌,并采用生理生化和16 SrDNA法对其进行鉴定,其次将腐败菌株进行回接试验,通过测定各腐败菌回接组的挥发性盐基氮(TVB-N)值、菌落数、感官评分判断其致腐能力。最后,采用滤纸片扩散法和营养肉汤法鉴定重组鲢鱼Cystatin(家族II)对优势腐败假单胞菌的抑菌效果。结果表明,荧光假单胞菌(Pseudomonas fluorescens)和草莓假单胞菌(Pseudomonas fragi)为主要致腐菌,所占比例分别为63.3%、26.7%,且荧光假单胞菌致腐能力强于草莓假单胞菌。重组Cystatin对两种菌均有明显的抑菌效果,且呈剂量依赖关系。

    Abstract:

    Investigation on the dominant spoilage pseudomonas from the chilled silver carp filets at the end of shelf life was carried out and then antibacterial experiment were conducted to determine the antibacterial activities of cystatin to the identified dominant spoilage pseudomonas. Firstly,the dominant spoilage Pseudomonas were isolated at the end of shelf life from the chilled silver carp fillets storaged at 4 ℃.Subsequently,physiological and biochemical method combined with 16S rRNA genes were conducted to identify these spoilage pseudomonas.After that,these strains were inoculated back to chilled silver carp filets at 4 ℃ and aerobic condition. TVB-N,the total number of bacterial colony combined with sensory evaluation were measured to evaluate the putrefying ability of these strains. Moreover,the experiments of filter paper diffusion and nutrient broth was both conducted to determine the antibacterial activities of cystatin to the identified dominant spoilage pseudomonas. The results in the present study demonstrated that the main putrefying strains were pseudomonas fluorescens and Pseudomonas fragi corresponding to the percent of 63.3% and 26.7% respectively. Pseudomonas fluorescens owned the stronger putrefying ability than Pseudomonas fragi. The antibacterial activity of the cystatin to the two dominant putrefying pseudomonas was dose-dependent.

    参考文献
    相似文献
    引证文献
引用本文

钟海霞,李冉,李树红,杨娟,胡强,柯勤勤,林灵,白稚子,李美良.重组Cystatin对冷藏鲢鱼的抑菌效果[J].食品与生物技术学报,2019,38(5):111-118.

ZHONG Haixia, LI Ran, LI Shuhong, YANG Juan, HU Qiang, KE Qinqin, LIN Ling, BAI Zhizi, LI Meiliang. Study on Bacteriostasis of the Cystatin from Chilled Silver Carp[J]. Journal of Food Science and Biotechnology,2019,38(5):111-118.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2019-07-26
  • 出版日期:

版权所有:《食品与生物技术学报》编辑部

地址:江苏省无锡市蠡湖大道1800号  邮政编码:214122

电话:0510-85913526  电子邮件:xbbjb@jiangnan.edu.cn

技术支持:北京勤云科技发展有限公司

微信公众号二维码

手机版网站二维码