乳酸乳球菌硫胺素转运蛋白ThiT提高酸耐受性的机制

doi:10.3969/j.issn.1673-1689.2020.09.003

首页 > 过刊浏览>2020年第39卷第9期 >16-24. DOI:10.3969/j.issn.1673-1689.2020.09.003

乳酸乳球菌硫胺素转运蛋白ThiT提高酸耐受性的机制
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                        10.3969/j.issn.1673-1689.2020.09.003
                    
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Mechanism of Thiamine Transporter ThiT Improving Acid Tolerance in Lactococcus lactis NZ9000

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摘要:

碳代谢引起的酸积累严重影响了微生物细胞的代谢活性。为提高细胞在酸性环境下的耐受性，通过过量表达硫胺素转运蛋白ThiT，研究了重组菌株的酸胁迫耐受性，并在此基础上通过比较转录组学对照菌株和重组菌株在正常和酸胁迫条件下关键基因的转录水平进行了研究。结果表明，经过酸胁迫（pH 4.0）处理后，重组菌株的最大存活率优势是对照菌株的16.2倍。转录组学的分析发现，过表达ThiT转运蛋白可提高细胞对碳水化合物的转运能力，从而为细胞抵御酸胁迫的耐受性提供更多的能量；此外寡肽、甜菜碱等相关转运基因的显著上调可帮助细胞抵御酸胁迫的损伤。作者探究了在乳酸乳球菌中过表达ThiT转运蛋白提高细胞酸胁迫耐受性的具体作用机制，为ThiT蛋白在其他工业微生物中的应用提供了理论基础。

Abstract:

The acid accumulation caused by carbon metabolism seriously affects the metabolic activity of microbial cells. In order to improve acid tolerance of cells, the acid tolerance of recombinant strain was studied by overexpression of the thiamine transporter ThiT. Based on this, comparative and recombinant strains were studied by comparative transcriptomics. We found that the survival rate of recombinant strain was 2.4 times higher than that of the control strain after 3 h of treatment with acid stress (pH 4.0). Transcriptomic analysis found that overexpression of the ThiT transporter increases the ability of cells to transport carbohydrates, thereby providing more energy for the cells against acid stress tolerance. In addition, significant upregulation of oligopeptides, betaine and other related transporters can help cells resist the damage of acid stress. The authors explored the specific mechanism of ThiT transporter in Lactococcus lactis to increasee tolerance of cellular acid stress. This study provided a theoretical basis for the application of ThiT protein in other industrial microorganisms.

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朱政明,张娟,堵国成,吴志猛.乳酸乳球菌硫胺素转运蛋白ThiT提高酸耐受性的机制[J].食品与生物技术学报,2020,39(9):16-24.

ZHU Zhengming, ZHANG Juan, DU Guocheng, WU Zhimeng. Mechanism of Thiamine Transporter ThiT Improving Acid Tolerance in Lactococcus lactis NZ9000[J]. Journal of Food Science and Biotechnology,2020,39(9):16-24.

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在线发布日期: 2020-11-26
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