不同粒径的免疫磁珠对食源性致病菌捕获效率的影响
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TS207.4

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Effect of the Size of Immunomagnetic Beads on Capture Efficiency for Foodborne Pathogenic Bacteria
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    摘要:

    粒径是影响免疫磁珠捕获效率的关键因素。选取微米级(1 μm)和亚微米级(300 nm)超顺磁珠,偶联沙门氏菌多克隆抗体制备免疫磁珠。通过流式细胞仪、分光光度计分别测定抗体偶联量和磁回收率。结果表明,与微米级免疫磁珠相比,亚微米级免疫磁珠抗体偶联量较高,在缓冲液中磁回收率较高,因此捕获率较高(300 nm: 55%,1 μm: 41%);在牛奶中磁回收率较低,导致捕获率明显降低(300 nm: 15%,1 μm: 26%)。因此,需根据待测样本的体积与黏度来确定免疫磁珠的粒径。

    Abstract:

    Particle size is a critical factor for capture efficiency (CE) of immunomagnetic beads (IMBs). IMBs were prepared by conjugating micrometer size (1 μm) or submicrometer size (300 nm)of superparamagnetic beads with anti- Salmonella polyclonal antibody. The amount of coupled antibody and the recovery rate were measured by flow cytometer and spectrophotometer, respectively. The results showed that compared with micron-IMBs, submicron-IMBs had a higher amount of coupled antibody and a higher recovery rate in buffer, and the CE was higher (300 nm: 55%, 1 μm: 41%). However, submicron-IMBs had a lower recovery rate in milk, which led to asignificant reduction of the CE of submicron-IMBs(300 nm: 15%, 1 μm: 26%). Therefore, the particle size of IMBs should be determined according to the volume and viscosity of samples.

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李静雯,杜美红,杨寅,陈婷,陈尔凝,赵瑞雪.不同粒径的免疫磁珠对食源性致病菌捕获效率的影响[J].食品与生物技术学报,2020,39(9):46-52.

LI Jingwen, DU Meihong, YANG Yin, CHEN Ting, CHEN Erning, ZHAO Ruixue. Effect of the Size of Immunomagnetic Beads on Capture Efficiency for Foodborne Pathogenic Bacteria[J]. Journal of Food Science and Biotechnology,2020,39(9):46-52.

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  • 在线发布日期: 2020-11-26
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