• 站内检索
    高级检索
  • 最新录用
    更多
文章摘要
反式肉桂醛对副溶血性弧菌的抑制作用
Antimicrobial Activity of Trans-cinnamaldehyde Against Vibrio parahaemolyticus
投稿时间:2017-10-07  修订日期:2017-11-10
DOI:
中文关键词: 反式肉桂醛  副溶血性弧菌  细胞膜完整性  鲜虾
英文关键词: trans-cinnamaldehyde  Vibrio parahaemolyticus  cell membrane integrity  fresh shrimp
基金项目:中央高校基本科研业务费专项资金资助
作者单位E-mail
郭都 西北农林科技大学食品科学与工程学院 duguo2911@163.com 
张文婷 西北农林科技大学食品科学与工程学院  
郝旭昇 西北农林科技大学食品科学与工程学院  
尹术华 西北农林科技大学食品科学与工程学院  
郭晓 西北农林科技大学食品科学与工程学院  
郑杨洋 西北农林科技大学食品科学与工程学院  
于海波 西北农林科技大学食品科学与工程学院  
石超 西北农林科技大学食品科学与工程学院 meilixinong@nwsuaf.edu.cn 
摘要点击次数: 71
全文下载次数: 
中文摘要:
      本文旨在探究植物源活性物质反式肉桂醛(trans-cinnamaldehyde,TC)对副溶血性弧菌的抑制作用及其对鲜虾食品中副溶血性弧菌的控制效果。首先试验通过测定TC对副溶血性弧菌的最小抑菌浓度(Minimum Inhibitory Concentration,MIC)评价其抑菌效果;随后通过测定TC对副溶血性弧菌生长曲线、生长动力模型、细胞膜完整性及细胞形态的影响探究其可能的抑菌机理;最后,构建副溶血型弧菌污染的鲜虾模型,评价TC对鲜虾中副溶血性弧菌的控制作用。结果表明,TC对副溶血性弧菌的MIC为50-70 μg/mL;TC可降低副溶血性弧菌最大生长速率、延长其生长延滞期;TC可使副溶血性弧菌细胞膜完整性显著降低,并使副溶血性弧菌细胞形态干瘪、皱缩;在鲜虾模型中,浓度为0.4%(v/v)的TC在1 h(4°C)使鲜虾中的副溶血性弧菌降低至检出限以下。本研究结果表明TC有潜力作为天然的抗菌物质应用于鲜虾及其他海产品中有效控制副溶血性弧菌。
英文摘要:
      The aim of this study was to investigate the antimicrobial activity of trans-cinnamaldehyde (TC) against V. parahaemolyticus. The minimum inhibitory concentration (MIC) of TC against V. parahaemolyticus was measured first. Then, the effects of TC on the bacterial growth curves, growth kinetic parameters, cell membrane integrity and cell morphology of V. parahaemolyticus were investigated. Moreover, the antimicrobial activity of TC against V. parahaemolyticus in shrimp samples was determined. The MICs of TC against 6 strains of V. parahaemolyticus were 50-70 μg/mL, TC resulted in a longer lag phase and lower growth rate of V. parahaemolyticus compared to the control. Electronic microscopy confirmed that cell membrane of V. parahaemolyticus was damaged by TC. In addition, 0.4% TC reduced the pathogen in shrimps to undetectable level in 1 h. These findings suggest that TC has potential to be developed as a natural preservative to control V. parahaemolyticus in shrimp or other seafood.
View Fulltext   查看/发表评论  下载PDF阅读器