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文章摘要
不同条件对纤维素糖化反应动力学参数的影响
Effect of The Kinetics of Cellulose Saccharification Under Different Conditions
投稿时间:2017-12-05  修订日期:2017-12-22
DOI:
中文关键词: 纤维素,水解,糖得率,动力学
英文关键词: cellulose, hydrolysis, sugar  yield, kinetics
基金项目:中国国家自然科学基金(31101269);江苏省自然科学基金(BK2011154)
作者单位E-mail
刘璐 农业生产加工工程研究所 1027033342@qq.com 
张志才 北京绿科天成生物有限公司
农业生产加工工程研究所
 
zhangzhicai@ujs.edu.cn 
樊亚娟 农业生产加工工程研究所  
胡坤雅 农业生产加工工程研究所  
汪佳 农业生产加工工程研究所  
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中文摘要:
      纤维素是秸秆中发酵生产生物燃料的主要成分,将纤维素转化为可发酵性糖是秸秆发酵合成生物燃料的关键步骤。以糖得率作为指标,研究了不同反应条件对纤维素糖化反应的动力学模型参数的影响。The Impeded Michaelis Model(IMM)能很好的拟合试验数据,模型包含酶与底物的可接近度(Kobs,0)和酶活性逐渐损失率(Ki)等两个参数。当pH 4.41,温度47 oC,纤维素酶浓度3%,底物浓度5%时,可获得糖得率Ytrs为46%,Kobs,0为0.2654,Ki为0.0583,与实验结果基本一致。温度和pH主要通过改变无效酶系数影响糖得率,而酶浓度和底物浓度主要改变酶对底物的可接性作用于糖得率;在试验浓度范围内Ytrs和Kobs,0呈正相关关系、和Ki成负相关关系,对木质纤维素的糖化研究奠定了基础。
英文摘要:
      The cellulose is the main component of the strain to produce bio-fuel. The saccharification of cellulose to fermentable sugars is critical step to fermentation of stalk to synthesize biofuel. The effects of various reaction conditions on parameters of the kinetics of cellulose saccharifications reaction were studied using the sugar yield (Ytrs) as an index. The Impeded Michaelis model containing two parameters including the accessibility between enzyme and the substrate (Kobs,0) and the gradual loss of enzyme activity (K i) could be fitted all experimental data well. The predicted maximum Ytrs (46%),Kobs,0(0.2654),Ki(0.0583)was obtained under the following conditions: pH of 4.41, temperature of 47 oC, 3% cellulase and 5% corn stover based on the model. The predicted results was in line with the experimental results. Temperature and pH mainly increase Ytrs by decreasing Ki, and the enzyme solubility and substrate concentration increases Ytrs mainly by increasing Kobs,0. Ytrs is significantly positively correlated with Kobs,0 and negatively correlated with Ki. This study lays the foundation for the saccharification of lignocellulose.
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