• 站内检索
    高级检索
  • 最新录用
    更多
文章摘要
不同溶剂同时蒸馏萃取艾叶挥发油的抑菌活性
Antimicrobial Activity of Artemisia argyi Essential Oils Extracted by Simultaneous Distillation in Different Solvent Systems
投稿时间:2018-04-01  修订日期:2018-05-04
DOI:
中文关键词: 同时蒸馏萃取  艾叶挥发油  扫描电镜(SEM)  抑菌活性
英文关键词: Simultaneous distillation extraction  Artemisia argyi Essential oil  Scanning Electron Microscope  Antimicrobial activity
基金项目:国家自然科学基金项目
作者单位E-mail
葛德鹏 上海理工大学 医疗器械与食品学院 gedepeng@hotmail.com 
李森 上海理工大学 医疗器械与食品学院  
黄凯 上海理工大学 医疗器械与食品学院  
管骁 上海理工大学 医疗器械与食品学院 gnxo@163.com 
摘要点击次数: 66
全文下载次数: 
中文摘要:
      采用二氯甲烷和正己烷两种溶剂,分别进行同时蒸馏萃取(SDE)工艺萃取艾叶挥发油,对得物进行气质联用(GC-MS)成分分析,测定其抑菌活性的差异,并通过扫描电镜(SEM)探究艾叶挥发油抑菌作用的机理。结果表明, SDE比水蒸气蒸馏萃取节省时间1 h,提取率达1.5%,是后者的3倍。SDE(正己烷)的得物抑菌活性更强,最低抑菌浓度集中在6.25 μL/mL,GC-MS结果可知其成分的92.95%是水蒸气蒸馏得物和SDE(二氯甲烷)的公共主要成分,SEM结果显示艾叶挥发油可作用于微生物细胞壁结构,从而达到抑制效果。
英文摘要:
      In this study, Artemisia argyi essential oil was extracted by simultaneous distillation (SDE) with dichloromethane and n-hexane, respectively. To explore the differences of antimicrobial activity, components of the oil were analyzed by GC-MS, and scanning electron microscope (SEM) was used to explore the mechanism of antimicrobial activity. Compared with the steam distillation, SDE had a three times higher yield (1.5%), and saved 1 hour. Results showed that SDE (n-hexane) oil had a higher antimicrobial activity, and the minimum inhibitory concentration (MIC) was 6.25 μL/mL. GC-MS results revealed that, the chemical constituents of the SDE (n-hexane) oil showed 92.95% similarity with the oil extracted with steam distillation and SDE (dichloromethane). SEM results suggested that antibacterial activity of Artemisia argyi essential oil could be achieved by disruption of cell wall.
View Fulltext   查看/发表评论  下载PDF阅读器