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文章摘要
一测多评法测定6种大豆异黄酮的含量
Quantitative Analysis of Six Soy Isoflavones by QAMS
投稿时间:2018-05-09  修订日期:2018-07-06
DOI:
中文关键词: 大豆异黄酮  一测多评  相对校正因子  高效液相色谱
英文关键词: Soybean isoflavone  quantitative analysis of multi-components by single-marker (QAMS)  relative correction factor (RCF)  high performance liquid chromatography
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
作者单位E-mail
李秀 江南大学食品学院 icebergxiu@163.com 
顾阳 江南大学食品学院  
杨燕 江南大学食品学院  
成向荣 江南大学食品学院 cheng-xiangrong@hotmail.com 
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中文摘要:
      目的:基于高效液相色谱紫外双波长检测方法,建立只用一个对照品同时测定6种大豆异黄酮含量的一测多评方法,以降低食品中大豆异黄酮含量测定的分析成本并提高分析效率。方法:样品经甲醇超声波辅助提取后,采用C18反相色谱柱;柱温25 ℃;进样量10 μL;检测波长262 nm和248 nm;流速1.0 mL/min;以0.2%甲酸水溶液和甲醇运行洗脱。通过一测多评分析确定6种大豆异黄酮的定量标准曲线、线性范围、相对校正因子、相对保留时间及相对峰面积,并对检测结果的精密度、稳定性、重复性、加样回收率以及系统的耐用性和适应性进行验证,且与外标法实验结果相比对。结果:6种大豆异黄酮在检测范围内均呈现出良好的线性关系(R2>0.9999),在不同品牌液相、色谱柱、柱温、流速条件下,大豆异黄酮的相对校正因子、相对保留时间、相对峰面积的RSD为0.06%~3.66%,线性范围内的样品在精密度、稳定性、重复性、加样回收率试验中RSD为0.85%~2.23%。一测多评法测定日常豆制品中6种大豆异黄酮的含量,并与外标法结果进行夹角余弦和相对误差分析。结果:表明两种方法测得的含量没有显著性差异,因此所建立的一测多评方法可用于大豆异黄酮的定量分析及相关产品的质量评价。
英文摘要:
      Objective: To establish a method for quantitative analysis of multi-components by single-marker (QAMS) of six soybean isoflavones by HPLC with ultraviolet detection of dual wavelength, which would decrease the cost of quantitative analysis of isoflavones and enhance the efficiency. Methods: The sample was extracted by methanol under the ultrasonic wave assistant, then analyzed by HPLC with a C18 column. The elution condition was 25 ℃, injection volume of 10μL, detection wavelength of 262 nm and 248 nm, elution rate of 1.0 mL/min with 0.2% formic acid in water and methanol. Six soybean isoflavones were used for the QAMS to obtain the standard curves, linear ranges, relative correction factors (RCFs), relative retention times, and relative peak areas. The precision, stability, repeatability, sample recovery rate, system durability and adaptability of QAMS method were tested and compared with those of external standard method. Results: Six soybean isoflavones showed good linearity within the analysis range (R2> 0.9999). In HPLC instruments from different manufacturers, chromatographic columns, column temperatures and flow rates, the RSD values of RCFs, relative retention times, and relative peak areas were in the range of 0.06% to 3.66%. The RSD values of precision, stability, reproducibility and sample recovery test were in the range of 0.85% to 2.23%. The isoflavone contents in daily bean products were detected by QAMS and external standard methods. The cosine of joint and relative errors of isoflavone contents analyzed by these two methods suggested no significant difference. Therefore, the established QAMS method for soybean isoflavones was successful in the quantitative analysis and quality evaluation for the related products.
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