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文章摘要
好氧堆肥用枯草芽孢杆菌GX2产芽孢工艺优化
Optimization of Sporulation Parameters for Aerobic Composting Bacillus Subtilis GX2 Strains
投稿时间:2019-06-11  修订日期:2019-08-19
DOI:
中文关键词: 堆肥用微生物菌剂  枯草芽孢杆菌  芽孢率  发酵工艺优化
英文关键词: Composting  microbial agents, Bacillus, Spore  rate, Fermentation  optimization
基金项目:国家重点研发计划课题(2016YFC0400707);水体污染控制与治理科技重大专项课题(2015ZX07306001)
作者单位E-mail
李月月 江南大学 环境与土木工程学院 1476025925@qq.com 
李秀芬 江南大学环境与土木工程学院 xfli@jiangnan.edu.cn 
齐希光 江南大学 食品学院  
李健 江南大学 环境科学与工程学院  
任月萍 江南大学 环境科学与工程学院  
王新华 江南大学 环境科学与工程学院  
夏琼琼 中国市政工程华北设计研究总院有限公司  
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中文摘要:
      芽孢杆菌是好氧堆肥微生物菌剂的重要组成部分,为降低菌剂生产成本、改善其品质及应用效果,对芽孢杆菌产芽孢工艺进行研究。首先,在摇瓶上进行枯草芽孢杆菌GX2的培养基及培养条件优化,在此基础上,在3 L发酵罐中进行分批补料优化研究,进一步提高细胞浓度,最后,通过单因素及正交试验研究,在3 L发酵罐中对产芽孢工艺进行了优化。结果表明,当pH为6.8、温度为50℃和搅拌转速为150 r/min时,发酵液中的活菌数达1.57×1010 CFU/mL,芽孢数达1.32×1010 CFU/mL,芽孢率为84.1%。堆肥用枯草芽孢杆菌GX2的发酵及产芽孢工艺优化可有效提高发酵液中的活菌数及芽胞率,降低菌剂的发酵及生产成本,为后续工业化生产及应用奠定基础。
英文摘要:
      Bacillus is an important microbial agent during the aerobic composting. To reduce the cost of bacteria production and improve bacteria quality and applicability, the spore production process of Bacillus was studied. Firstly, the culture medium and environmental conditions of Bacillus Subtilis GX2 were optimized using shaking flasks. Cell concentration was increased by a batch feed optimization method in a 3 L fermenter. Finally, single-factor and orthogonal experiments were applied to the spore-forming process to find its optimized conditions . The results showed that the number of viable cells and spores in the fermentation broth reached 1.57×1010 CFU/mL and 1.32×1010 CFU/mL with a spore rate of 84.1% at pH of 6.8, temperature of 50°C and the rotation speed of 150 r/min, respectively. To conclude, the optimization of fermentation and spore production for Bacillus Subtilis GX2 effectively increased the number of viable cells and spores in the fermentation broth, and reduced the cost of microbial agents. This work provides some guidance for the culture of Bacillus and lays a foundation for the researches on aerobic composting.
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