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文章摘要
粒径对免疫磁珠食源性致病菌捕获效率的影响
Effect of Immunomagnetic Beads Size on Capture Efficiency of Foodborne Pathogenic Bacteria
投稿时间:2019-06-20  修订日期:2019-08-04
DOI:
中文关键词: 微米级免疫磁珠  亚微米级免疫磁珠  粒径  食源性致病菌  捕获效率  磁回收率
英文关键词: Micron-immunomagnetic beads  Submicron-immunomagnetic beads  Particle size  Foodborne pathogen  Capture efficiency  Recovery rate
基金项目:北京市科学技术研究院创新团队计划项目(IG201807C1);国家重点研发计划资助项目(2016YFF0203802)
作者单位E-mail
李静雯 北京市理化分析测试中心 li.j.w@hotmail.com 
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中文摘要:
      粒径是影响免疫磁珠捕获效率的关键因素。选取微米级(1 μm)和亚微米级(300 nm)超顺磁珠,偶联沙门氏菌多克隆抗体制备免疫磁珠。通过流式细胞仪、分光光度计分别测定抗体偶联量和磁回收率。结果表明,与微米级免疫磁珠相比,亚微米级免疫磁珠抗体偶联量较高,在缓冲液中磁回收率较高,因此捕获率较高(300 nm: 55%,1 μm: 41%);在牛奶中磁回收率较低,导致捕获率明显降低(300 nm: 15%,1 μm: 26%)。因此,需根据待测样本的体积与粘度来确定免疫磁珠的粒径。
英文摘要:
      Particle size is a critical factor for capture efficiency (CE) of immunomagnetic beads (IMBs). IMBs were prepared by using micrometer size (1 μm) and submicrometer size (300 nm) superparamagnetic beads, which were conjugated with anti-Salmonella polyclonal antibody. The amount of coupled antibody and the recovery rate were measured by flow cytometer and spectrophotometer, respectively. The results showed that compared with micron-IMBs, submicron-IMBs had a higher amount of coupled antibody and a higher recovery rate in buffer, so the CE was higher (300 nm: 55%%, 1 μm: 41%). However, submicron-IMBs had a lower recovery rate in milk, which led to the significant reduction of the CE of submicron-IMBs (300 nm: 15%, 1 μm: 26%). Therefore, the particle size of IMBs should be determined according to the volume and viscosity of samples.
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