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文章摘要
基于上转换纳米颗粒和金纳米颗粒的Cd2+免疫检测新方法
A Novel Immunoassay Method for Cd2+ Based on Up-conversion Nanoparticles and Gold Nanoparticles
投稿时间:2019-11-22  修订日期:2019-11-22
DOI:
中文关键词: 荧光共振能量转移  上转换纳米颗粒  金纳米颗粒  
英文关键词: fluorescence resonance energy transfer  up-conversion nanoparticles  gold nanoparticles  cadmium
基金项目:国家重点研发计划(2016YFF0203701/2016YFF0103800);国家自然科学基金(31401566);粮食深加工与品质控制湖南省2011协同创新项目(2013448);常熟市科技发展计划(社会发展类)项目(CS201605);苏州市科技计划项目(SNG201617)
作者单位E-mail
邓晨 长沙理工大学 lilin1340717142@163.com 
李琳 长沙理工大学  
张林威 长沙理工大学  
程云辉 长沙理工大学  
陈茂龙 长沙理工大学  
许宙 长沙理工大学 xz_jnu@126.com 
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中文摘要:
      基于上转换纳米颗粒(UCNPs)和金纳米颗粒(Au NPs)间的荧光共振能量转移(FRET)和抗原抗体的识别作用构建了Cd2+的免疫检测平台。制备水溶性UCNPs表面修饰Cd-抗原作为能量供体探针,同时在AuNPs表面修饰Cd-抗体作为能量受体探针。抗原-抗体的免疫结合使得UCNPs和AuNPs发生FRET过程,引起UCNPs荧光猝灭;当检测体系中存在Cd2+时,Cd2+与UCNPs-抗原竞争性地结合AuNPs-抗体,从而抑制了FRET过程,荧光信号值随着Cd2+含量的增加而增加。结果表明,该方法检测范围为0.01~10 ng/mL,检出限可达0.01 ng/mL。将该方法应用于自来水样品中Cd2+的检测,当加标水平为0.1、1、10 ng/mL时,回收率为98%-109%,相对标准偏差为3.4%-4.1%。
英文摘要:
      A immunoassay platform for Cd2+was constructed based on fluorescence resonance energy transfer(FRET) between the up-conversion nanoparticles(UCNPs) and the gold nanoparticles(AuNPs) and antigen-antibody recognition. Water-soluble UCNPs modified with Cd-antigen were prepared as an energy donor probe, AuNPs modified by Cd-antibody were synthesized as an energy receptor probe. The immune binding of antigen-antibody causes FRET process of UCNPs and AuNPs, which causes fluorescence quenching of UCNPs; when Cd2+ is present in the detection system, Cd2+ competitively binds to AuNPs-antibody, thereby inhibiting the FRET process, and the fluorescence signal value increases with the increase of Cd2+ content. The results showed that the detection range of this method ranged from 0.01 ng/mL to10 ng/mL, and the detection limit was 0.01 ng/mL. It was applied to the detection of Cd2+ in tap water samples. At the spiked level was 0.1, 1, 10 ng / mL, the recoveries for Cd2+ ranged from 98% to 109%, with the relative standard deviations(RSDs) of 3.4% to 4.1%.
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