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文章摘要
苍术挥发油杀菌活性评价及抑菌机制研究
Study on The Bactericidal Activity and Antibacterial Mechanism of Volatile Oil from Atractylodes Chinensis Koidz
投稿时间:2019-12-10  修订日期:2020-01-27
DOI:
中文关键词: 苍术  挥发油  杀菌活性  杀菌机制
英文关键词: Atractylodes chinensis  Volatile oil  bactericidal activity  germicidal efficacy
基金项目:
作者单位E-mail
王喆 北京城市学院 keliaopeituola@126.com 
蒋圆婷 北京城市学院  
靳羽含 北京城市学院  
赵晨宇 北京城市学院  
刘艳丽 北京城市学院  
梁寒峭 北京城市学院 13691241519@163.com 
马国需 中国医学科学院 药用植物研究所  
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中文摘要:
      目的:研究苍术挥发油体外杀菌活性及抑菌机制。方法:以大肠埃希氏菌、金黄色葡萄球菌、白假丝酵母为供试菌,采用悬液定量杀菌实验测定苍术挥发油的杀菌活性,并测定对三种供试菌胞外碱性磷酸酶(AKP)、可溶性蛋白含量和生长曲线的影响,从而阐述苍术挥发油的杀菌机制。结果:苍术挥发油对三种常见致病菌均有显著的抑制或杀灭作用。苍术挥发油作用后,三种致病菌悬液中AKP、可溶性蛋白质等大分子物质含量比对照组显著增加,经苍术挥发油处理过的三种菌生长受到明显抑制。结论:苍术挥发油能够通过改变菌体细胞膜通透性,破坏菌体完整结构发挥体外杀菌活性。
英文摘要:
      Objective : To evaluate the bactericidal activity in vitro and study the antibacterial mechanism of Atractylodes chinensis volatile oil. Method The antibacterial activity against Escherichia coli, Staphylococcus aureus and Candida albicans was evaluated by the suspension quantitative germicidal test. The antibacterial mechanism was studied by leakage rate of extracellular alkaline phosphatase (AKP) , soluble protein content and growth curve. Results The results showed that volatile oil from A. chinensis was effective against the tested microorganism. The contents of alkaline phosphatase (AKP) and soluble protein were increased obviously when compared with those in the control group, while the growth of indicators were obviously suppressed. Conclusions The A. chinensis volatile oil showed prominent bactericidal activity and the mechanism maybe lies in cell membrane permeability changed and cell integrity damaged.
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