Recombinant Expression and Whole-Cell Immobilization of Tagatose 4-Epimerase from Thermotoga petrophila in Escherichia coli

doi:10.12441/spyswjs.20230211003

Home > Archive>Volume 43, Issue 11, 2024 >106-114. DOI:10.12441/spyswjs.20230211003

Recombinant Expression and Whole-Cell Immobilization of Tagatose 4-Epimerase from Thermotoga petrophila in Escherichia coli
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                        10.12441/spyswjs.20230211003
                    
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Affiliation:1.School of Biotechnology, Jiangnan University, Wuxi 214122, China;2.Key Laboratory of Industrial Biotechnology Ministry of Education, Jiangnan University, Wuxi 214122, China;3.State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China;4.International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China
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Abstract:

D-tagatose is a rare sugar with multiple physiological functions, but its current biological production methods face challenges such as high substrate costs and unstable supply. After molecular modification, the tagatoronate 3-epimerase from Thermotoga petrophila can synthesize D-tagatose through epimerization reaction with D-fructose as substrate, and this mutant is denoted as tagatose 4-epimerase (T4E). This study successfully expressed T4E in Escherichia coli BL21 (DE3) through fusion of T7 tag to its N-terminal, characterized its enzymatic properties, and optimized the bioconversion conditions for the synthesis of D-tagatose from D-fructose catalyzed by T4E. Meanwhile, the immobilization of recombinant E. coli BL21 (DE3) cells expressing heterologous T4E using diatomaceous earth-sodium alginate was studied. The results showed that under optimal conditions, the equilibrium conversion rate could reach 25% after 4 h of reaction with 300 g/L D-fructose as the substrate. Compared with the T4E in free cells, the whole-cell immobilized T4E exhibited significantly improved thermal stability, enabling continuous reaction for seven batches and demonstrating high industrial application potential.

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GUO Xuehong, LIU Zhanzhi, XU Ying, WU Jing. Recombinant Expression and Whole-Cell Immobilization of Tagatose 4-Epimerase from Thermotoga petrophila in Escherichia coli[J]. Journal of Food Science and Biotechnology,2024,43(11):106-114.

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Received:February 11,2023
Revised:March 07,2023
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Online: February 21,2025
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