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Home > Archive>Volume 43, Issue 11, 2024 >44-59. DOI:10.12441/spyswjs.20240315002
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Progress on Screening and Application of ssDNA Aptamers for Foodborne Pathogens
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1.School of Yunkang Medicine and Health, Nanfang College, Guangzhou 510970, China;2.Biomedical Big Data Center, Nanfang College, Guangzhou 510970, China

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    Abstract:

    Single-stranded DNA (ssDNA) aptamer is a short single-stranded deoxyribonucleotide sequence obtained by the systematic evolution of ligands by exponential enrichment (SELEX) in vitro. It can achieve high affinity and specific binding to targets through conformational changes and possesses the advantages of simple preparation, low cost, relative stability, ease of modification, and low immunogenicity, earning it the name “chemical antibodies”. ssDNA aptamers have shown great potential in the field of food safety detection. This paper reviewed the ssDNA aptamer screening technology based on SELEX, its application in the detection of foodborne pathogens, current research limitations and potential future development directions. The aim is to provide a theoretical basis for improving food safety supervision and the prevention and control of foodborne disease.

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ZHANG Ming, YIN Zhenzhen, WU Hengzhi. Progress on Screening and Application of ssDNA Aptamers for Foodborne Pathogens[J]. Journal of Food Science and Biotechnology,2024,43(11):44-59.

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History
  • Received:March 15,2024
  • Revised:April 29,2024
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  • Online: February 21,2025
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