Abstract:This study investigated the effect of medium compositions and the environmental conditions on the production of antitumor metabolite and listed as follows: carbon source,corn starch,nitrogen source,yeast extract;the ratio of carbon and nitrogen,12:6;If 5 mg/L Gly,Glu,Ala,10 mg/L Try,Tyr,1.0 mg/L LiSO4、MnCl2、Na2MoO4、NaH2PO4 were supplemented to the culture broth,the yield of antitumor secondary metabolite would be significant improved.The optimum fermentation conditions were as follows: fermentation term,7 d;initiative pH 7.4;fermentation temperature,30 ℃;seed culture term,4 d;the volume of culture liquid contained in 500 mL conical tank,125 mL;rotor speed,150 r/min.
