白藜芦醇对酪氨酸酶体外抑制活性的动力学研究
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中央高校基本科研业务费专项资金资助项目(TD2010-3、BLYX200935);第38批留学回国人员科研启动基金

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    摘要:

    在30℃,pH 6.8的Na2HPO4-NaH2PO4的缓冲体系中,采用酶促动力学方法,研究了白藜芦醇对酪氨酸酶单酚酶和二酚酶的抑制作用。结果表明,白藜芦醇对酪氨酸酶、单酚酶和二酚酶均有抑制作用,对单酚酶抑制活性的IC50值(抑制率达到50%时的白藜芦醇质量浓度)约为5.1mg/mL,对二酚酶抑制活性的IC50值约为5.6 mg/mL。此外,白藜芦醇可延长单酚酶的迟滞效应,8 mg/mL的白藜芦醇能使迟滞时间从22 s延长至62 s,而对二酚酶则无此迟滞作用。Lineweav-er-Burk图分析表明,白藜芦醇对酪氨酸酶的抑制作用为混合型抑制,对游离酶的抑制常数(KI)和对酶-底物络合物的抑制常数(KIS)分别为3.4 mg/mL和35.98 mg/mL。

    Abstract:

    The inhibitory effect of resveratrol on the activity of monophenolase and diphenolase existing in tyrosinase was investigated by the enzymatic kinetic method at 30 ℃ in a Na2HPO4-NaH2PO4 buffer system(pH6.8).The results indicated that resveratrol could inhibit both the monophenolase and diphenolase activities of tyrosinase,and the IC50 values(resveratrol concentration corresponding to 50% inhibitory rate) were 5.1 mg/mL and 5.6 mg/mL,respectively.Furthermore,resveratrol could extent the lag time of monophenolase for oxidation of L-tyrosine,of which 8 mg/mL of resveratrol prolonged the lag time from 22 s to 62 s,while no such effect was observed on diphenolase.According to the Lineweaver-Burk plot,resveratrol was found to be a mixed inhibitor for the oxidation of L-DOPA,and the equilibrium constants for binding with free enzyme KI and with enzyme-substrate complex KIS were 3.4 mg/mL and 35.98 mg/mL,respectively.

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杨阳,鲁军,孙冰洁,王丹,尹晓燕,任迪峰.白藜芦醇对酪氨酸酶体外抑制活性的动力学研究[J].食品与生物技术学报,2011,30(4):632-635.

YANG Yang, LU Jun, SUN Bing-jie, WANG Dan, YIN Xiao-yan, REN Di-feng. Inhibitory Kinetics Study of Resveratrol on Tyrosinase Activity in Vitro[J]. Journal of Food Science and Biotechnology,2011,30(4):632-635.

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  • 在线发布日期: 2014-06-17
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