Abstract:To study the possible mechanisms of Pomegranate Peel Polyphenols、Punicalagine and ellagic acid on cholesterol biosynthesis.The model of steatotic L-02 hepatocyte was established by cultured with 50%(V/V) fetal bovine serum for 48 hours,and the appropriate concentrations of PPPs were detected by MTT assay,Then the experiment is divided into there groups:normal group、 model group and therapy group,different contents of PPPs is added to the therapy group.After 48h cultured,the lipid droplets stained with oil red O were observed,meanwhile,the intracellular total cholesterol levels were determined by HPLC and the activity of HMG-CoA reductase was detected by ultraviolet spectro-velocity method.The accumulation of lipid droplets and the content of TC were decreased in PPPs treatment group,existing dose-effect relationship,meanwhile,the activity of HMG-CoA reductase was significantly inhibited in PPPs treatment groups,especially in the Punicalagin group.PPPs can decrease the content of TC in steatotic hepatocytes,The possible mechanism is by downregulating the activity of HMG-CoA reductase,Punicalagin was the key component in PPPs on lipid-lowering.
