Abstract:To observe the effects of different fermentation days of Shuanqian Junzhi(SQJZ,a Strychnos nux-vomica product fermented by Trametes cinnabarina) on acute toxicity and analgesic,anti-inflammatory activity,seven samples of different fermentation days(0 d,7 d,20 d,41 d,48 d,62 d and 69 d) were applied.The acute toxicity and anti-inflammatory,analgesic activities of SQJZ of different fermentation days were evaluated in animal models.Listed as followed was the acute toxicity of different SQJZ in a descending order:0 d≈7 d <20 d≈41 d <48 d <62 d <69 d.The sustaining twisting reaction times was:69 d≈62 d>48 d>41 d≈20 d>0 d>7 d.According to the control group,the twisting times caused by acetic acid of the crude,0 d,7 d,20 d,41 d,48 d of SQJZ noticeably decreased(P<0.05) just like the crude Strychnos nux-vomica,which indicated the analgesic action of the crude did not change significantly within 48 day-fermentation period.However,62 d and 69 d of SQJZ had no significant analgesic effect and there was a significant difference(P<0.01) in comparison with the positive drug indometacin,which showed a complete loss of the anti-inflammatory effect after 62 day-fermentation.The swelling percent decrease of external ear was:7 d≈20 d>41 d>48 d>0 d>>62 d>69 d.With the control group,0 d,7 d,20 d,41 d and 48 d of SQJZ were able to reduce the swelling of murine external ear caused by croton oil(P<0.05 or 0.01),the same as the crude,indicating that their anti-inflammatory effect did not declined through 48 day-fermentation.Therefore,the feasible period of fermentation of SQJZ is about 41-48 days.
