离子交换法纯化α-酮戊二酸

doi:10.3969/j.issn.1673-1689.2013.10.006

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离子交换法纯化α-酮戊二酸
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                        10.3969/j.issn.1673-1689.2013.10.006
                    
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基金项目:国家自然科学基金项目(31171638)

Ion-Exchang Purificatioon of Alpha-Ketoglutaric Acid in Fermentation Broth

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为了从发酵液中分离纯化出α-酮戊二酸,作者利用活性炭对发酵液进行脱色后,采用离子交换法对α-酮戊二酸的纯化工艺进行了研究。结果表明,活性炭用量1.5 g/L、pH 3.0、脱色时间1 h、脱色温度50℃的条件下能取得最佳脱色效果,脱色率达89.2%。分析了6种阴离子交换树脂对α-酮戊二酸的吸附容量,确定了吸附性能显著的D301大孔弱碱型树脂。在固定床上上样条件优化表明,当发酵液中α-酮戊二酸质量浓度为20 g/L、上样流速为2.67 BV/h、径高比为1∶12时,上样吸附效果最佳。采取阶段洗脱工艺:第一阶段用0.01 mol/L的NaCl和0.01 mol/L的HCl混合溶液进行洗脱,第二阶段采用0.3 mol/L的HCl溶液在6 BV/h的流速下进行洗脱。验证结果表明,在此洗脱工艺下,收集液中α-酮戊二酸与丙酮酸的质量比由初始发酵液中的1.5∶1提高为10∶1时,α-酮戊二酸收率为93.2%。因此,采用离子交换法可有效的分离纯化发酵液中的α-酮戊二酸。

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In order to purify α-ketoglutarate acid from fermentation broth,the purification technology of α-ketoglutarate acid by ion-exchange method was studied after decolored by activated carbon.The results showed as follows. Firstly,the decolorization ratio reached 89.2% under the following optimal conditions:carbon dosage 1.5 g/L,pH 3,bleaching time 1 h,bleaching temperature50 ℃. Subsequently,the process of ion-exchange was studied and among 6 different resins D301weakly-based anion resin was the optimal one for α-ketoglutaric acid abstraction by comparison of the adsorption capacity. And the optimal absorption efficiency was achieved when the α-ketoglutarate acid concentration in the fermentation broth was 20 g/L,the sample flow rate was 2.67BV/h and the diameter to height ratio was 1 ∶12. Finally,a process of stepwise elution wasinvestigated. In the first step,0.01 mol/L NaCl and 0.01 mol/L HCl mixed solution was used as eluent and in the second step,0.3 mol/L HCl was used as eluent and the flow rate was 6 BV/h.With the elution strategy above,the final yield of α-ketoglutaric acid reached 93.2% and an excellent purification result was achieved with the increased mass ratio of α-ketoglutaric acid to pyruvic acid from 1.5∶1 to 10∶1. Therefore,ion-exchange method was valid to purify α-ketoglutaric acid from fermatation borth.

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占宏德,李江华,刘龙,堵国成,陈坚.离子交换法纯化α-酮戊二酸[J].食品与生物技术学报,2013,32(10):1043-1048.

ZHAN Hong-de, LI Jiang-hua, LIU Long, DU Guo-cheng, CHEN Jian. Ion-Exchang Purificatioon of Alpha-Ketoglutaric Acid in Fermentation Broth[J]. Journal of Food Science and Biotechnology,2013,32(10):1043-1048.

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