Abstract:The polysaccharide from Chinese Venus was firstly separated through acolumn of DEAE-cellulose(Whatman DE 52). As a result,three independent elution peaks(F1,F2and F3)were obtained. The three fractions were loaded onto a column of Sephadex G-100,affording f1,f2and f3,respectively. Purity of f1,f2and f3was further confirmed by using HPLC,and results showed that f1,f2and f3were homogeneous polysaccharides respectively. The chemical characterizations of polysaccharides were investigated by various methods. As to CP,f1,f2and f3,polysaccharides content,measured by employing the method of sulfuric acid-phenol coloration,was 83.81%,98.75%,95.58% and 84.71%,respectively. Uronic acid content,determined by using the method of sulfate-3-phenylphenylol assay,was 1.58%,0.16%,0.96% and 2.13%,respectively. Sulfuric radical content,determined by employing the method of gelatin-barium chloride assay,was 0.92%,1.22%,2.08% and 3.58%,respectively. Protein content,measured by applying the method of coomassie brilliant blue coloration,was 3.08%,not detected,not detected and 6.34% respectively. The relative molecular weight of f1,f2and f3,determined by HPLC,was 68 600,80 600 and 100 600,respectively. The CP,f1,f2and f3had different reducing power,lipid peroxidation inhibition effect and ferrous ion chelating activity.
