新疆阿魏菇抗氧化肽分离纯化及抗氧化性质

doi:10.3969/j.issn.1673-1689.2015.05.013

首页 > 过刊浏览>2015年第34卷第5期 >524-529. DOI:10.3969/j.issn.1673-1689.2015.05.013

新疆阿魏菇抗氧化肽分离纯化及抗氧化性质
DOI:
                        10.3969/j.issn.1673-1689.2015.05.013
                    
CSTR:
                        [cstr]
                    
作者:
                        
                        
                    
作者单位:
作者简介:
通讯作者:
中图分类号:
基金项目:

Separation, Purification and Antioxidant Activity Study of Antioxidant Peptides from Pleurotus ferulae lenzi in Xinjiang

Author:

Affiliation:

Fund Project:

摘要

图/表

访问统计

参考文献

相似文献

引证文献

资源附件

文章评论

摘要:

采用超滤法、离子交换层析和凝胶过滤层析分离阿魏菇抗氧化多肽,研究其抗氧化性能。以胃蛋白酶-胰蛋白酶联合酶解阿魏菇多肽水解物为原料,采用超滤法分离阿魏菇水解液,使用10 000的超滤膜,将阿魏菇水解液分离成10 000以下的多肽组分和大于10 000的多肽组分,小于10 000的多肽组分的·OH自由基清除能力和DPPH·自由基清除能力较高。小于10 000的多肽组分经DEAE-32纤维素分离,得到3个洗脱峰,其中P3组分的羟基清除能力较高,达到50.22%。P3组分采用葡聚糖凝胶SephadexG-25分离,在220 nm处得到5个洗脱峰,组分P3-2的羟自由基清除最高,达到77.23%,具有较高的抗氧化活性,其相对分子质量大约为1 862。

Abstract:

Antioxidant peptides of Pleurotus ferulae lenzi were separated using ultra-filtration,ion-exchange and gel filtration chromatography,and its antioxidant properties were also studied in this study. Two kinds of peptides(＞10 000 polypeptide components and ＜10 000 polypeptide components )were isolated from the hydrolyzed solution of Pleurotus ferulae lenzi protein. The polypeptide components under 10 000 have higher ·OH radical scavenging and DPPH· radical scavenging capacity than those above 10 000. After detaching of polypeptide components under 10 000 by DEAE-32,three elution peaks were obtained and P3 component showed higher hydroxyl scavenging capacity,reaching 50.22%. Five elution peaks at 220 nm were further isolated from P3 component by Sephadex G-25,and P3-2 component showed best capacity of ·OH radical scavenging,reaching 77.23%. According to standard equation,the molecular weight of P3-2 was about 1 862. The results provide a theoretical basis for further studies of active ingredient from Pleurotus ferulae lenzi in Xinjiang province.

参考文献

相似文献

引证文献

引用本文

郑晓吉,许程剑,牛博楠,陈帅,王雪铭,朱丽莉.新疆阿魏菇抗氧化肽分离纯化及抗氧化性质[J].食品与生物技术学报,2015,34(5):524-529.

ZHENG Xiaoji, XU Chengjian, NIU Bonan, CHEN Shuai, WANG Xueming, ZHU Lili. Separation, Purification and Antioxidant Activity Study of Antioxidant Peptides from Pleurotus ferulae lenzi in Xinjiang[J]. Journal of Food Science and Biotechnology,2015,34(5):524-529.

复制

文章指标

点击次数:
下载次数:
HTML阅读次数:
引用次数:

历史

收稿日期:
最后修改日期:
录用日期:
在线发布日期: 2015-08-09
出版日期:

文章二维码

地址：江苏省无锡市蠡湖大道1800号邮政编码：214122

电话：0510-85913526 电子邮件：xbbjb@jiangnan.edu.cn

技术支持：北京勤云科技发展有限公司

引用本文

分享

文章指标

历史

文章二维码

微信公众号二维码

手机版网站二维码