Abstract:A rapid detection method of hydrogen peroxide (H2O2) was established, which was based on the optical signal of gold nanoparticles(AuNPs) generated. With the synergetic effect of 4-(2-Hydroxyethyl)-1- piperazineethanesulfonic acid(HEPES), sodium citrate and polyvinylpyrrolidone (PVP), H2O2 reduced chloroauric acid and AuNPs of different colors were generated with different concentrations of H2O2. H2O2 could thus be qualitatively detected by the naked eyes, and the sensitivity of 0.8 μmol/L was reached. By the spectrophotometry, 0.06 μmol/L of H2O2 could be detected with the linear range of 0.2~1 000 μmol/L. Using the method, the beef omasum samples spiked with 10, 25 and 100 μg/g H2O2 were analyzed with the detection recoveries of 75.2%~82.5% and relative deviation of less than 13%. Moreover, by the naked eyes 25 μg/g of H2O2 in beef omasum could be detected. The developed method is therefore highly sensitive, low cost, convenient and rapid and has high application potential in the H2O2 detection for various foods and biological samples.
