重组大肠杆菌生产海藻糖合酶发酵工艺优化
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Optimization of Trehalose Synthase Fermentation Conditions from Recombinant Escherichia coli
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    摘要:

    为了实现来源于嗜热栖热菌的海藻糖合酶的高效表达,对E.coli BL21(DE3)/pET24a(+)-TreS基因工程菌在3 L发酵罐进行了发酵工艺优化,获得的最优发酵条件为:诱导温度32 ℃,当OD600达到50时以0.2 g/(L·h)的速率补加乳糖进行诱导产酶,在该条件下发酵35 h时酶活达到472 U/mL,是优化前的2.3倍;进而采用该条件在30 L发酵罐进行了初步放大研究,酶活达到356 U/mL。由于该海藻糖合酶热稳定性好,因此尝试了高温处理破碎E.coli细胞以释放海藻糖合酶,结果表明,80 ℃处理30 min,海藻糖合酶的得率可达72.3%,具有工业化应用前景。

    Abstract:

    In order to achieve efficient production of the trehalose synthase from Thermus thermophilus,the optimization of fermentation conditions was investigated in E.coli BL21(DE3) harboring the plasmid pET24a(+)-TreS. The optimized fermentation conditions were as follows:the inducing temperature is 32 ℃,the adding rate of lactose is 0.2 g/(L·h) When OD600 reached 50. Under the condition,the enzyme activity reached 472 U/mL at cultivation of 35 h,was 2.3 times than before. In addition,the fermentation was performed in 30 L bioreactor under the condition of preceding narrative,the enzyme activity reached 356 U/mL. Because the thermal stability of trehalose synthase is good, E.coli cell was broken up through high temperature. The results showed that treated it 30 min under 80 ℃ the yield of trehalose synthase reached 72.3%. It have industrial application prospect.

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张悦,宿玲恰,吴敬.重组大肠杆菌生产海藻糖合酶发酵工艺优化[J].食品与生物技术学报,2016,35(9):913-919.

ZHANG Yue, SU Lingqia, WU Jing. Optimization of Trehalose Synthase Fermentation Conditions from Recombinant Escherichia coli[J]. Journal of Food Science and Biotechnology,2016,35(9):913-919.

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  • 在线发布日期: 2016-11-01
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