壳聚糖和pH对CHO细胞收获液澄清效率的影响

doi:10.3969/j.issn.1673-1689.2017.03.015

首页 > 过刊浏览>2017年第36卷第3期 >322-326. DOI:10.3969/j.issn.1673-1689.2017.03.015

壳聚糖和pH对CHO细胞收获液澄清效率的影响
DOI:
                        10.3969/j.issn.1673-1689.2017.03.015
                    
作者:
                        
                        
                    
作者单位:
作者简介:
通讯作者:
中图分类号:TS202.3
基金项目:

Effect of Chitosan and pH on Clarification Process Efficiency of Harvested CHO Cell Culture Fluid

Author:

Affiliation:

Fund Project:

摘要

图/表

访问统计

参考文献

相似文献

引证文献

资源附件

文章评论

摘要:

作者研究了壳聚糖和pH对中国仓鼠卵巢细胞（Chinese hamster ovary，CHO）培养液料液澄清过程的影响。初步探索了在CHO细胞收获液（pH 7.0）中添加0~0.10 g/dL壳聚糖对0.22 μm膜过滤通量的影响。结果显示，添加0.06 g/dL壳聚糖时膜过滤通量最大，达到8 266.22 L/（m2·h）。探索了pH值（5.0~7.0）对收获液澄清效率的影响，研究发现，不添加壳聚糖的情况下降低培养液pH值对膜过滤通量没有十分显著的提高。为更多地去除宿主细胞蛋白（Host Cell Protein，HCP）和残留DNA，作者研究了pH值与壳聚糖对膜过滤通量及残留杂质的交互影响。结果表明，pH为5.0壳聚糖质量浓度为0.06 g/dL时滤出液中残留DNA几乎完全去除，HCP含量降低50%以上，而抗体的回收率不受影响。

Abstract:

Chitosan is a natural，non-toxic and biodegradable flocculant. This study focused on determining and optimizing the clarification process efficiency in the presence of various concentrations （0~ 0.10 g/dL） of chitosan at different pH 5.0~7.0 for the harvested Chinese hamster ovary （CHO） cell culture fluid （HCCF）（pH 7.0）. Following HCCF was treated with 0.06 g/dL chitosan，the filtration flux was achieved up to 8 266.22 L/（m2·h） using 0.22 μm filter. In addition，the result showed that similar filtration flux for HCCF at different pH without chitosan addition whereas increasing filtration flux was achieved when HCCF pre-treated with chitosan at decreasing pH. To further improve this clarification process，the combined effect of various concentrations of chitosan at different pH on the filtration flux and the removal of process impurities including host cell proteins （HCP） and residual DNA in HCCF was evaluated. The result of a full factorial experiment including two factors and three levels （chitosan at 0.02，0.04，0.06% and pH at 5，6，7） showed that 90% antibody product yield with nearly complete removal of residual DNA and 50% more reduction of HCP were obtained when HCCF pre-treated with the optimal 0.06 g/dL chitosan at pH 5.0.

参考文献

相似文献

引证文献

引用本文

翁源灿,杨彬,程习文,鄢成伟,孙文正.壳聚糖和pH对CHO细胞收获液澄清效率的影响[J].食品与生物技术学报,2017,36(3):322-326.

WENG Yuancan, YANG Bin, CHENG Xiwen, YAN Chengwei, SUN Wenzheng. Effect of Chitosan and pH on Clarification Process Efficiency of Harvested CHO Cell Culture Fluid[J]. Journal of Food Science and Biotechnology,2017,36(3):322-326.

复制

文章指标

点击次数:
下载次数:
HTML阅读次数:
引用次数:

历史

收稿日期:
最后修改日期:
录用日期:
在线发布日期: 2017-04-28
出版日期:

文章二维码

地址：江苏省无锡市蠡湖大道1800号邮政编码：214122

电话：0510-85913526 电子邮件：xbbjb@jiangnan.edu.cn

技术支持：北京勤云科技发展有限公司

引用本文

分享

文章指标

历史

文章二维码

微信公众号二维码

手机版网站二维码