褐藻胶降解过程及链剪切模型的研究
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Degradation Process and Chain Scission Models of Alginate During Enzymatic Hydrolysis
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    利用多角度激光光散射检测器(GPC-MALLS)研究了褐藻胶裂解酶(alginate lyase)和纤维素酶(Cellulase)一步法和分步法降解方式下褐藻胶产物相对分子质量参数的变化,分析并确定了链剪切模型及低相对分子质量褐藻胶降解条件。结果表明Cellulase无法单独作用于褐藻胶。alginate lyase表现为多次剪切模型和中央剪切模型,alginate lyase分步降解法(iA)提高了酶利用率。alginate lyase降解条件:一步法反应控制在2 h内,分步法控制在5 h内,酶添加量为2.56~10.24 U/mg。分步法中进一步探究了alginate lyase和Cellulase的协同作用,加酶间隔时间为0 min(iACS),30 min(iACM),60 min(iACE)。结果表明:alginate lyase主要作用于Mw≥1×105的相对分子质量组分,Cellulase主要作用于1×104~1×105的相对分子质量组分。iACE法中产物PDI保持稳定且无限趋近于2,呈现出随机剪切模型。iACS和iACM法中使产物相对分子质量组分增加,PDI值上升至5.14和7.02。

    Abstract:

    The changes of molecular weight parameters of alginates hydrolyzed by alginate lyase (ALyase) and cellulase using one-step and step-by-step methods were studied by GPC-MALLS. The chain shear models and the degradation conditions were determined. The results showed cellulase had no function on alginate if used alone. Multiple shear model and central shear model were observed when alginate lyase used. Furthermore, the utilization rate was improved with the step-by-step method of alginate lyase. The duration of one-step and step-by-step degradation using 2.56~10.24 U/mg alginate lyase were with in 2 h and 5 h, respectively. The synergistic effect of alginate lyase with cellulase was further explored by step-by-step method at 0 min (iACS), 30 min (iACM), 60 min (iACE). Alginate lyase preferred to take effect on the alginates of molecular weight (Mw)≥1×105, while cellulase preferred to 1×104~1×105 g/mol. The PDI values were stable and approached to 2 infinitely in the iACE method, showing a random shear model. In the iACS and iACM methods, the hydrolyzed products varied in components with increased molecular weight, and their PDI values rose to 5.14 and 7.02 respectively.

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陈佳薇,陈静静,张涛,江波.褐藻胶降解过程及链剪切模型的研究[J].食品与生物技术学报,2021,40(1):43-50.

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  • 在线发布日期: 2021-04-07

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