一株产CoQ<sub>10</sub>的类球红细菌分离鉴定及诱变选育

doi:10.3969/j.issn.1673-1689.2021.02.005

首页 > 过刊浏览>2021年第40卷第2期 >32-40. DOI:10.3969/j.issn.1673-1689.2021.02.005

一株产CoQ10的类球红细菌分离鉴定及诱变选育
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                        10.3969/j.issn.1673-1689.2021.02.005
                    
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Isolation, Identification and Mutation of a Rhodobacter sphaeroides with High Yield of Coenzyme Q₁₀

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辅酶Q₁₀（CoQ₁₀）是天然存在的抗氧化剂和细胞代谢激活剂，具有消除自由基、维持细胞膜通透性、提高机体免疫功能等多种生理作用。选育CoQ₁₀高产菌株并通过发酵法实现该功能化合物的高效合成具有重要价值。作者从污水处理厂的淤泥中分离纯化获得了一株CoQ₁₀高产菌株YLL-13，通过形态观察、生化特性分析及16S rDNA序列比对，鉴定为类球红细菌（Rhodobacter sphaeroides），命名为R. sphaeroides YLL-13。随后，以R. sphaeroides YLL-13为出发菌株，以维生素K3和4-羟基苯甲酸（p-hydroxybenzoic acid，pHBA）为复合筛选标记，通过亚硝基胍化学诱变，筛选高产突变菌株，并初步研究了其生产CoQ₁₀特性。结果显示，经诱变选育获取了一株遗传稳定的CoQ₁₀高产突变株R.sphaeroides YLL-13-T，其具有相对低类胡萝卜素合成、高生长速率和高CoQ₁₀积累特性。在所考察的发酵体系中，发酵120 h后突变菌株YLL-13-T的生物量、CoQ₁₀产量和CoQ₁₀产率达到83.5、1.04 g/L和12.46 mg/g，比出发菌株分别提高了12.3%、42.5%和23.6%，展现出良好的工业化应用前景。

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Coenzyme Q ₁₀(CoQ₁₀) is a natural antioxidant and cell metabolic activator, possessing several important biological properties, such as eliminating free radicals, maintaining cell membrane permeability, and improving immune function. It is of great value to develop high-yield CoQ₁₀ strain and realize high-efficient synthesis of functional compounds by fermentation. In this work, a potent CoQ₁₀-producing strain YLL-13 was isolated from the silt of a sewage treatment plant. Based on the morphological observation, biochemical characteristics analysis and 16S rDNA sequence alignment, the strain was identified as Rhodobacter sphaeroides and named as R. sphaeroides YLL-13. In further, a high-yield mutant strain was screened by nitrosoguanidine chemical mutagenesisusing R. sphaeroides Yll-13 as the starting strainand Vitamin K3 and p-hydroxybenzoic acid(pHBA) as the compound screening markers. A genetically stable CoQ₁₀mutant with high yield of CoQ₁₀, R. sphaeroides YLL-13-T,was achieved by mutagenesis. This strain had relatively low carotenoid synthesis, high growth rate and high CoQ10 accumulation characteristics. In the studied fermentation system, the biomass, CoQ₁₀ concentration and specific CoQ₁₀ content of the mutant strain YLL-13-T after 120 h fermentation were 83.5 g/L, 1.04 g/L and 12.46 mg/g(DCW) respectively, which were 12.3%, 42.5% and 23.6% higher than those of original wild-type strain, showing a good prospect of industrial application.

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姚丽莉,许齐纯,刘晓萌,李鸿鑫,高鑫誉,徐梧皓,吕常江,曹宏伟.一株产CoQ₁₀的类球红细菌分离鉴定及诱变选育[J].食品与生物技术学报,2021,40(2):32-40.

YAO Lili, XU Qichun, LIU Xiaomeng, LI Hongxin, GAO Xinyu, XU Wuhao, LYU Changjiang, CAO Hongwei. Isolation, Identification and Mutation of a Rhodobacter sphaeroides with High Yield of Coenzyme Q₁₀[J]. Journal of Food Science and Biotechnology,2021,40(2):32-40.

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在线发布日期: 2021-05-28
出版日期: 2021-02-15

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