黑曲霉降解黄曲霉毒素B<sub>1</sub>的研究及转录组分析

doi:10.3969/j.issn.1673-1689.2021.09.003

首页 > 过刊浏览>2021年第40卷第9期 >16-25. DOI:10.3969/j.issn.1673-1689.2021.09.003

黑曲霉降解黄曲霉毒素B1的研究及转录组分析
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                        10.3969/j.issn.1673-1689.2021.09.003
                    
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Biodegradation of Aflatoxin B₁ by Aspergillus niger and Transcriptome Analysis

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为探究黑曲霉FS10对AFB₁的降解机制，使用黑曲霉FS10的不同组分（菌悬液、发酵液、孢子、菌丝体）对AFB₁进行降解，并研究了AFB₁刺激对黑曲霉FS10降解效果的影响；利用扫描电子显微镜观察降解过程中黑曲霉菌丝体的形态变化；用转录组学技术探究AFB₁可能的降解机理。结果表明：黑曲霉FS10能有效降解AFB₁，72 h时菌悬液的对AFB₁脱除率高达98.65%；黑曲霉FS10孢子对AFB₁无明显脱除作用，但菌丝体对AFB₁有一定的吸附能力，发酵液对AFB₁有明显脱除效果；经过AFB₁诱导刺激后黑曲霉FS10降解效果有明显提升，表明AFB₁处理能显著提升黑曲霉FS10对AFB₁的降解能力。微观结构分析表明AFB₁处理在一定程度上影响黑曲霉FS10的形态，但随着时间的延长这种影响逐渐减小。此外，转录组学分析表明AFB₁处理降低了一些能量代谢基因的水平，这可能是黑曲霉FS10的一种自我保护机制，同时蛋氨酸的合成基因上调，推测AFB₁的降解可能与蛋氨酸的合成有关。

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Among the known mycotoxins, aflatoxin B₁ (AFB₁) is the most toxic, and its impact on food safety has caused widespread concern. To explore the degradation mechanism of AFB1 by Aspergillus niger FS10, different fractions of A.niger FS10(suspension, fermentation, spores, mycelium) were used to degrade AFB₁. The effects of AFB₁ stimulation on the degradation of A.niger FS10 were studied and the morphological changes of A.niger FS10 mycelium during the degradation process were observed under scanning electron microscope. Finally, the possible mechanism of AFB₁ degradation was explored by transcriptome technology. The results showed that AFB₁ could be effectively degraded by A.niger FS10, and the removal rate of AFB₁ was 98.65% after 72 h treatment. The spores of A.niger FS10 insignificantly affected the AFB₁ removal, but the mycelium had a certain adsorption capacity for AFB₁ and the fermentation broth had obvious effect on the removal of AFB1. After the induced stimulation of AFB₁, the degradation effect of A.niger FS10 was significantly improved, indicating that AFB₁ treatment could significantly improve the degradation ability of A.niger FS10 to AFB₁. The microstructure analysis showed that AFB₁ treatment affected the morphology of A.niger FS10 to a certain extent, but this effect gradually decreased with the treated time. Moreover, the transcriptomic analysis showed that AFB₁ treatment decreased the levels of some energy metabolism-related genes, which might be a self-protection mechanism of A.niger FS10. And the up-regulation of methionine synthesis genes suggested that the degradation of AFB1 might be related to the methionine synthesis process.

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杨阳,邱天宇,袁晓,孙嘉笛,张银志,孙秀兰,纪剑.黑曲霉降解黄曲霉毒素B₁的研究及转录组分析[J].食品与生物技术学报,2021,40(9):16-25.

YANG Yang, QIU Tianyu, YUAN Xiao, SUN Jiadi, ZHANG Yinzhi, SUN Xiulan, JI Jian. Biodegradation of Aflatoxin B₁ by Aspergillus niger and Transcriptome Analysis[J]. Journal of Food Science and Biotechnology,2021,40(9):16-25.

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在线发布日期: 2021-09-28
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