不同米蛋白组分与铅的结合规律

doi:10.3969/j.issn.1673-1689.2021.12.007

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不同米蛋白组分与铅的结合规律
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                        10.3969/j.issn.1673-1689.2021.12.007
                    
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Study on Binding Behaviors of Lead to Different Insoluble Rice Proteins

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为了探究不同米蛋白组分与重金属铅（Pb²⁺）的结合规律，从动力学、热力学角度对3种水不溶性米蛋白（球蛋白、谷蛋白、醇溶蛋白）与Pb²⁺的结合特征进行了分析。结果表明：3种蛋白质与Pb²⁺的结合均很迅速，30 min即可达到反应平衡；醇溶蛋白对Pb²⁺的结合能力最强，平衡结合量达20.54 mg/g；米蛋白与Pb²⁺的结合过程符合准二级动力学模型，Langmuir等温吸附模型的拟合效果（R²=0.980~0.995）要优于Freundlich模型（R²=0.847~0.987），说明两者结合以化学吸附为主；热力学参数ΔS°> 0、ΔH°> 0、ΔG°< 0，说明3种米蛋白与Pb²⁺的结合均为自发、熵驱动的吸热反应。X射线光电子能谱（XPS）表明醇溶蛋白与Pb²⁺结合后，N1s和S2p的特征峰强度显著降低（P<0.05），说明Pb²⁺主要与醇溶蛋白中的含氮、含硫基团相结合。扫描电镜（SEM）显示醇溶蛋白与Pb²⁺结合后，颗粒发生聚集，呈致密团块状结构，进一步验证了Pb²⁺和醇溶蛋白的结合。

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The binding characteristics of three water-insoluble rice proteins(globulin, glutenin, prolamin) and Pb²⁺ were studied from the perspective of kinetics and thermodynamics to explore the binding behaviors of different rice protein components and heavy metal lead ion(Pb²⁺). The result showed that Pb²⁺ was rapidly bound to each insoluble rice protein, and the binding equilibrium was reached within 30 min. The prolamin showed the highest binding capacity with a binding adsorption quantity(q) of 20.54 mg/g. The binding of Pb²⁺ to rice proteins was consistent with the quasi-second-order kinetic model, and the Langmuir equation(R²=0.980~0.995) had greater fitting effect than that of the Freundlich equation(R²=0.847~0.987). The result indicated the interaction between protein subunits and Pb²⁺ was mainly by chemical absorptions. The thermodynamic parameters, i.e., ΔS°>0, ΔH°>0 and ΔG°<0, indicated the spontaneous, entropy-driven and endothermic binding reaction. X-ray photoelectron spectroscopy(XPS) showed that the characteristic peak intensity of N1s and S2p was significantly decreased after binding (P< 0.05), suggesting that the major binding sites of prolamin for Pb²⁺ were nitrogen- and sulfur-containing groups. Scanning electron microscopy (SEM) displayed that the prolamin structures aggregated and formed a compact cloddy structure after binding to Pb²⁺, which further verified the binding of Pb²⁺ to rice prolamin.

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冯伟,范大明,董田田,李克强,倪双双,王涛,张昊,王韧.不同米蛋白组分与铅的结合规律[J].食品与生物技术学报,2021,40(12):52-58.

FENG Wei, FAN Daming, DONG Tiantian, LI Keqiang, NI Shuangshuang, WANG Tao, ZHANG Hao, WANG Ren. Study on Binding Behaviors of Lead to Different Insoluble Rice Proteins[J]. Journal of Food Science and Biotechnology,2021,40(12):52-58.

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在线发布日期: 2021-12-27
出版日期: 2021-12-25

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