环丙沙星降解菌的筛选及其降解特性探究

doi:10.3969/j.issn.1673-1689.2021.12.008

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环丙沙星降解菌的筛选及其降解特性探究
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                        10.3969/j.issn.1673-1689.2021.12.008
                    
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Screening of Ciprofloxacin Degrading Bacteria and Its Degradation Characteristics

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摘要:

为获取一株有效降解环丙沙星的菌株，并探究其在降解过程中的降解特性，利用高效液相色谱法筛选环丙沙星降解菌，根据菌落形态、生理生化特性以及16S rDNA基因序列分析，对降解菌进行鉴定；并研究菌株活性、胞内外物质以及细胞壁对质量浓度为4 μg/mL环丙沙星的降解作用。结果表明，罗伊氏乳杆菌WQ-Y1在物理吸附和生物降解的共同作用下，降解率最高可达70.2%，其胞内物质、胞外物质以及细胞壁对环丙沙星的降解率分别为17.7%、23.4%和37.0%，远低于活菌的降解率。可见罗伊氏乳杆菌WQ-Y1对环丙沙星的物理吸附作用和菌株体内的酶都并非是菌株降解环丙沙星的单一途径，而是依赖于完整的菌株活性。

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In order to screena strain that could effectively degrade ciprofloxacinand to explore its degradation characteristics in the degradation process, high performance liquid chromatography was employed to screen ciprofloxacin degrading bacteria. This bacterium was then identified according to the colony morphology, physiological and biochemical characteristics as well as the 16S rDNA gene sequence analysis. The strain activity, and the degradation of intracellular and extracellular substances and cell walls on ciprofloxacin were also investigated with 4 μg/mL ciprofloxacin. The results showed that the degradation rate of ciprofloxacin by the screened Lactobacillus reuteri WQ-Y1 strain reached to 70.2% via physical adsorption and biodegradation. Furthermore, the degradation rates of ciprofloxacin by intracellular substances, extracellular substances and cell walls were 17.7%, 23.4% and 37.0%, respectively, which were far lower than that of the living bacteria. It could be concluded that the physical adsorption and the enzymolysis process of L. reuteri WQ-Y1 were not the only way of ciprofloxacin degradation, and it was also dependenton the complete liability of L. reuteri WQ-Y1.

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瞿春晓,温健竹,蔡振东,潘道东,吴振.环丙沙星降解菌的筛选及其降解特性探究[J].食品与生物技术学报,2021,40(12):59-64.

QU Chunxiao, WEN Jianzhu, CAI Zhendong, PAN Daodong, WU Zhen. Screening of Ciprofloxacin Degrading Bacteria and Its Degradation Characteristics[J]. Journal of Food Science and Biotechnology,2021,40(12):59-64.

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在线发布日期: 2021-12-27
出版日期: 2021-12-25

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