基于比色和荧光双信号检测戊唑醇的免疫层析试纸条的构建

doi:10.3969/j.issn.1673-1689.2022.01.005

首页 > 过刊浏览>2022年第41卷第1期 >36-43. DOI:10.3969/j.issn.1673-1689.2022.01.005

基于比色和荧光双信号检测戊唑醇的免疫层析试纸条的构建
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                        10.3969/j.issn.1673-1689.2022.01.005
                    
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Fabrication of Immunochromatography Strip for Tebuconazole Based on Colorimetric and Fluorescent Dual-Signal Detection

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摘要:

基于Au@PDA核壳纳米粒子和其对量子点荧光淬灭效应，制备了一种双信号读取模式的免疫层析试纸条，可用于定性或定量检测果蔬中戊唑醇的含量。研究了纳米金和抗体复合物的制备、试纸条传感元件的载量和缓冲液等因素对试纸条检测灵敏度的影响。在最优条件下，采用比色检测模式时，所构建双信号戊唑醇试纸条，其肉眼定性检测限为1 μg/mL；而采用荧光检测模式时，其肉眼定性检测限为100 ng/mL。将该试纸条应用于黄瓜样品检测，结果显示：黄瓜样品的提取液通过适当稀释后，样品基质的干扰可以消除；试纸条对黄瓜样品的检测限为0.8 mg/kg，可满足国家安全标准的限量要求。

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Based on Au@PDA core-shell nanoparticles (NPs) and the fluorescence quenching toward quantum dots, a dual-signal readout mode lateral flow immunoassay strip (LFIA) was fabricated for qualitative or quantitative determination of tebuconazole in fruits and vegetables. The effects of the preparation of Au@PDA and Au@PDA-antibody complex, the load of sensing elements and buffers of the strip were investigated on the strip sensitivity. Under the optimal conditions, the qualitative detection limit of tebuconazole by the naked eyes was 1 μg/mL in colorimetric mode and 100 ng/mL in fluorescent detection mode. The developed strip was applied in the tebuconazole detection in cucumber samples. And the results showed the interference of sample matrix could be eliminated by an appropriate dilution of cucumber sample extract. The limit of detection for tebuconazole in cucumber was 0.8 mg/kg, meeting the limit requirement of national safety standard.

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赵赟,蔡伊娜,张存政,彭池方.基于比色和荧光双信号检测戊唑醇的免疫层析试纸条的构建[J].食品与生物技术学报,2022,41(1):36-43.

ZHAO Yun, CAI Yina, ZHANG Cunzheng, PENG Chifang. Fabrication of Immunochromatography Strip for Tebuconazole Based on Colorimetric and Fluorescent Dual-Signal Detection[J]. Journal of Food Science and Biotechnology,2022,41(1):36-43.

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在线发布日期: 2022-02-21
出版日期: 2022-01-25

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