好氧反硝化芽孢杆菌JD-014的分离鉴定及脱氮性能

doi:10.3969/j.issn.1673-1689.2022.01.009

首页 > 过刊浏览>2022年第41卷第1期 >68-76. DOI:10.3969/j.issn.1673-1689.2022.01.009

好氧反硝化芽孢杆菌JD-014的分离鉴定及脱氮性能
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                        10.3969/j.issn.1673-1689.2022.01.009
                    
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Isolation, Identification and Nitrogen Removal Characteristics of Aerobic Denitrifying Bacillus Strain JD-014

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为了更好地实现以绿色环保的方式处理食品工业废水中的氮素污染物，从某河道水体中分离筛选出了1株具有好氧反硝化功能的芽孢杆菌JD-014。通过对该菌株在脱氮过程中气态氮（N₂）的产生、氮平衡估算以及酶活测定证实了菌株JD-014的好氧反硝化途径，并对影响其脱氮性能的环境因素进行了研究。结果表明：以50 mg/L NO₃^--N为唯一氮源时，菌株JD-014对NO₃^--N的去除速率为0.56 mg/（L·h）。在脱氮过程中，该菌株可将NO₃^--N部分转化为N₂并伴随有相关功能酶活的检出；当碳源为丁二酸钠和柠檬酸钠、碳氮比（C/N）为10~25、温度为37 ℃、转速为150~200 r/min、盐度为1%以下时，该菌株脱氮性能最佳。此外，菌株JD-014还具有较好的亚硝酸盐耐受性，在NO₂^--N质量浓度为10~200 mg/L时，脱氮率可保持在46.62%~99.92%。本研究表明，好氧反硝化芽孢杆菌JD-014在实际食品加工废水的氮素污染生物处理方面具有很大的应用潜力。

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A strain of Bacillus subtilis JD-014 with aerobic denitrification function was isolated and screened out from a river course in this study to treat and remove the nitrogen pollutants in food industrial wastewater in a green and environmentally friendly way. The aerobic denitrification pathway within this strain was verified by the gaseous nitrogen production, the nitrogen balance calculation and the functional enzymatic activity measurement during the nitrogen removal process. The effect of different environmental factors on its nitrogen removal performance was investigated as well. The results showed that the nitrogen removal rate of JD-014 was 0.56 mg/（L·h） when sodium nitrate (50 mg/L) was used as the sole nitrogen source. The strain was found to partly transfer the NO₃^--N into N₂ accompanied by the detection of related functional enzyme activity. The optimal conditions for JD-014 to achieve the best nitrogen removal performance were carbon source of sodium succinate or sodium citrate, C/N ratio of 10 to 25, temperature of 37 ℃, shaking speed of 150 to 200 r/min, and salinity of less than 1%. Moreover, JD-014 had great nitrite tolerance, and the nitrogen removal efficacy could be kept within 46.62%~99.92% when the concentration of NO₂^--N was 10~200 mg/L. These results suggested that the aerobic denitrifying B. subtilis JD-014 had great potential in the biological treatment of nitrogen pollution in food processing wastewater.

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杨婷,辛瑜,时祎,张梁,石孔泉,张丽娜.好氧反硝化芽孢杆菌JD-014的分离鉴定及脱氮性能[J].食品与生物技术学报,2022,41(1):68-76.

YANG Ting, XIN Yu, SHI Yi, ZHANG Liang, SHI Kongquan, ZHANG Lina. Isolation, Identification and Nitrogen Removal Characteristics of Aerobic Denitrifying Bacillus Strain JD-014[J]. Journal of Food Science and Biotechnology,2022,41(1):68-76.

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在线发布日期: 2022-02-21
出版日期: 2022-01-25

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