Abstract:The magnetic covalent organic framework was synthesized quickly and easily by the co-precipitation method and characterized by Fourier transform infrared spectroscopy and transmission electron microscopy. A new method for the determination of benzo[a]pyrene (BaP) in roast meat by magnetic solid phase extraction-high performance liquid chromatography was developed based on the optimization of experimental conditions such as adsorbent dose, extraction time, desorption solvent, desorption time and volume. The linear range of the method was 0.5~100.0 μg/L (R2=0.999 8), the limit of detection (S/N=3) and the limit of quantitation (S/N=10) were 0.14 μg/L and 0.47 μg/L, respectively. The relative standard deviations (RSDs) of the intra-day (n=5) and inter-day(n=5) were 3.5% and 3.9%, respectively. On this basis, this method was successfully applied to the determination of BaP in roast meats (grilled fish, beef and mutton) and BaP was detected in all three samples. The recoveries were in the range from 76.0% to 123.4% at three spiked concentrations (10.0, 20.0, 30.0 ng/g) by standard addition method, and the relative standard deviation was 1.7%~ 10.6%.
