人参皂苷Rg1对疲劳小鼠骨骼肌中TFAM和NRF-1基因表达的影响

doi:10.3969/j.issn.1673-1689.2022.02.009

首页 > 过刊浏览>2022年第41卷第2期 >67-72. DOI:10.3969/j.issn.1673-1689.2022.02.009

人参皂苷Rg1对疲劳小鼠骨骼肌中TFAM和NRF-1基因表达的影响
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                        10.3969/j.issn.1673-1689.2022.02.009
                    
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Effects of Ginsenoside Rg1 on Gene Expression of TFAM and NRF-1 in Skeletal Muscle of Fatigued Mice

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为了研究人参皂苷 Rg1 对疲劳模型小鼠抗疲劳作用及骨骼肌中线粒体转录因子 A（TFAM）和核呼吸因子-1（NRF-1）基因表达的影响，选取 70 只雄性 BALB/c 小鼠随机分为7组，除空白组外，小鼠腹腔注射环磷酰胺，造成免疫低下模型后进行小鼠负重游泳力竭实验。ELISA 法测定小鼠肝脏及骨骼肌中疲劳相关指标的变化情况，RT-PCR 法检测小鼠骨骼肌中 TFAM 和 NRF-1 基因表达水平。结果表明，人参皂苷 Rg1 中剂量组小鼠负重游泳的力竭时间最长，较空白组增加了 271.4%，且能显著增加肝糖原浓度。各剂量组小鼠肝脏 MDA 质量摩尔浓度下降，LDH 活性升高。中剂量人参皂苷 Rg1 可显著增加小鼠骨骼肌中 TFAM mRNA 和 NRF-1 mRNA 表达水平。人参皂苷 Rg1 可有效调节疲劳小鼠骨骼肌中疲劳相关生化指标的水平，具有较好的抗疲劳效果。

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The anti-fatigue effect of ginsenoside Rg1 on fatigue model mice and its influence on mitochondrial transcription factor A (TFAM) and nuclear respiratory factor-1 (NRF-1) gene expression in skeletal muscle were investigated. Seventy male BALB/c mice were randomly divided into seven groups. Besides the blank group, mice were injected intraperitoneally with cyclophosphamide to create an immunodeficiency model. And then the mice were subjected to the full-load swimming exhaustion experiment. Changes in fatigue-related indexes in liver and skeletal muscle of mice were determined by ELISA. The expression levels of TFAM and NRF-1 genes in the skeletal muscle of mice were detected by RT-PCR. The results showed that the duration of weight-bearing swimming was the longest in the middle dose group of ginsenoside Rg1, which increased by 271.4% compared with that of the blank group. The content of liver glycogen was significantly increased. The level of MDA in liver of mice in each dose group decreased, while the level of LDH increased. Medium dose of ginsenoside Rg1 could significantly increase the expression of TFAM mRNA and NRF-1 mRNA levels in the skeletal muscles of mice. Ginsenoside Rg1 could effectively adjust the fatigue-related biochemical indicators in skeletal muscle of fatigue mice, exhibiting better anti-fatigue effect.

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孔凡秀,杨琪,董佳萍,谢琳琳,王鹤霖,迟晓星.人参皂苷Rg1对疲劳小鼠骨骼肌中TFAM和NRF-1基因表达的影响[J].食品与生物技术学报,2022,41(2):67-72.

KONG Fanxiu, YANG Qi, DONG Jiaping, XIE Linlin, WANG Helin, CHI Xiaoxing. Effects of Ginsenoside Rg1 on Gene Expression of TFAM and NRF-1 in Skeletal Muscle of Fatigued Mice[J]. Journal of Food Science and Biotechnology,2022,41(2):67-72.

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在线发布日期: 2022-03-01
出版日期: 2022-02-25

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